Local production of lipoprotein-associated phospholipase A2 and lysophosphatidylcholine in the coronary circulation: Association with early coronary atherosclerosis and endothelial dysfunction in humans

Shahar Lavi, Joseph P. McConnell, Charanjit S. Rihal, Abhiram Prasad, Verghese Mathew, Lilach O Lerman, Amir Lerman

Research output: Contribution to journalArticle

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Abstract

BACKGROUND - Lipoprotein-associated phospholipase A2 (Lp-PLA2) is a novel marker and participant in vascular inflammation. Inflammation also is associated with coronary atherosclerosis. We tested the hypothesis that local coronary production of Lp-PLA2 is enhanced in patients with early coronary atherosclerosis and associated with local endothelial function. METHODS AND RESULTS - Coronary angiography, blood flow, flow reserve, endothelial function assessment, and intravascular ultrasound with volumetric analysis were performed in 15 patients with mild coronary atherosclerosis and in 15 control subjects. Plasma samples were collected simultaneously from the left main coronary artery and coronary sinus for measurement of Lp-PLA2, lysophosphatidylcholine (a product of Lp-PLA2), and C-reactive protein. Hemodynamic parameters and cholesterol were similar in both groups. Arterial Lp-PLA2 levels were similar in patients and control subjects: 225 ng/mL (interquartile range [IQR], 196 to 273 ng/mL) versus 221 ng/mL (IQR, 177 to 294 ng/mL). Lp-PLA2 net production in the coronary circulation was higher in patients compared with control subjects: 519 ng/min (IQR, 198 to 1276 ng/min) versus -529 ng/min (IQR, -872 to -79 ng/min; P=0.001) and correlated with percent atheroma volume (rs=0.37, P=0.04). Net production of lysophosphatidylcholine was higher in patients compared with control subjects: 199 ng/min (IQR, -592 to 470 ng/min) versus -505 ng/min (IQR, -1119 to 0 ng/min; P=0.03) and correlated with coronary endothelial dysfunction (rs=0.5, P=0.005). C-reactive protein was not significantly different between the groups. CONCLUSIONS - Early coronary atherosclerosis in humans is characterized by local production of Lp-PLA2. Local coronary production of lysophosphatidylcholine, the active product of Lp-PLA2, is associated with endothelial dysfunction. These results support the role for Lp-PLA2 in the mechanism of regional vascular inflammation and atherosclerosis in humans.

Original languageEnglish (US)
Pages (from-to)2715-2721
Number of pages7
JournalCirculation
Volume115
Issue number21
DOIs
StatePublished - May 2007

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1-Alkyl-2-acetylglycerophosphocholine Esterase
Coronary Circulation
Lysophosphatidylcholines
Coronary Artery Disease
Inflammation
C-Reactive Protein
Blood Vessels
Coronary Sinus
Type C Phospholipases
Atherosclerotic Plaques
Coronary Angiography
Atherosclerosis
Coronary Vessels
Hemodynamics
Cholesterol

Keywords

  • Atherosclerosis
  • Endothelium
  • Inflammation
  • Vasculature

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Local production of lipoprotein-associated phospholipase A2 and lysophosphatidylcholine in the coronary circulation : Association with early coronary atherosclerosis and endothelial dysfunction in humans. / Lavi, Shahar; McConnell, Joseph P.; Rihal, Charanjit S.; Prasad, Abhiram; Mathew, Verghese; Lerman, Lilach O; Lerman, Amir.

In: Circulation, Vol. 115, No. 21, 05.2007, p. 2715-2721.

Research output: Contribution to journalArticle

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title = "Local production of lipoprotein-associated phospholipase A2 and lysophosphatidylcholine in the coronary circulation: Association with early coronary atherosclerosis and endothelial dysfunction in humans",
abstract = "BACKGROUND - Lipoprotein-associated phospholipase A2 (Lp-PLA2) is a novel marker and participant in vascular inflammation. Inflammation also is associated with coronary atherosclerosis. We tested the hypothesis that local coronary production of Lp-PLA2 is enhanced in patients with early coronary atherosclerosis and associated with local endothelial function. METHODS AND RESULTS - Coronary angiography, blood flow, flow reserve, endothelial function assessment, and intravascular ultrasound with volumetric analysis were performed in 15 patients with mild coronary atherosclerosis and in 15 control subjects. Plasma samples were collected simultaneously from the left main coronary artery and coronary sinus for measurement of Lp-PLA2, lysophosphatidylcholine (a product of Lp-PLA2), and C-reactive protein. Hemodynamic parameters and cholesterol were similar in both groups. Arterial Lp-PLA2 levels were similar in patients and control subjects: 225 ng/mL (interquartile range [IQR], 196 to 273 ng/mL) versus 221 ng/mL (IQR, 177 to 294 ng/mL). Lp-PLA2 net production in the coronary circulation was higher in patients compared with control subjects: 519 ng/min (IQR, 198 to 1276 ng/min) versus -529 ng/min (IQR, -872 to -79 ng/min; P=0.001) and correlated with percent atheroma volume (rs=0.37, P=0.04). Net production of lysophosphatidylcholine was higher in patients compared with control subjects: 199 ng/min (IQR, -592 to 470 ng/min) versus -505 ng/min (IQR, -1119 to 0 ng/min; P=0.03) and correlated with coronary endothelial dysfunction (rs=0.5, P=0.005). C-reactive protein was not significantly different between the groups. CONCLUSIONS - Early coronary atherosclerosis in humans is characterized by local production of Lp-PLA2. Local coronary production of lysophosphatidylcholine, the active product of Lp-PLA2, is associated with endothelial dysfunction. These results support the role for Lp-PLA2 in the mechanism of regional vascular inflammation and atherosclerosis in humans.",
keywords = "Atherosclerosis, Endothelium, Inflammation, Vasculature",
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T1 - Local production of lipoprotein-associated phospholipase A2 and lysophosphatidylcholine in the coronary circulation

T2 - Association with early coronary atherosclerosis and endothelial dysfunction in humans

AU - Lavi, Shahar

AU - McConnell, Joseph P.

AU - Rihal, Charanjit S.

AU - Prasad, Abhiram

AU - Mathew, Verghese

AU - Lerman, Lilach O

AU - Lerman, Amir

PY - 2007/5

Y1 - 2007/5

N2 - BACKGROUND - Lipoprotein-associated phospholipase A2 (Lp-PLA2) is a novel marker and participant in vascular inflammation. Inflammation also is associated with coronary atherosclerosis. We tested the hypothesis that local coronary production of Lp-PLA2 is enhanced in patients with early coronary atherosclerosis and associated with local endothelial function. METHODS AND RESULTS - Coronary angiography, blood flow, flow reserve, endothelial function assessment, and intravascular ultrasound with volumetric analysis were performed in 15 patients with mild coronary atherosclerosis and in 15 control subjects. Plasma samples were collected simultaneously from the left main coronary artery and coronary sinus for measurement of Lp-PLA2, lysophosphatidylcholine (a product of Lp-PLA2), and C-reactive protein. Hemodynamic parameters and cholesterol were similar in both groups. Arterial Lp-PLA2 levels were similar in patients and control subjects: 225 ng/mL (interquartile range [IQR], 196 to 273 ng/mL) versus 221 ng/mL (IQR, 177 to 294 ng/mL). Lp-PLA2 net production in the coronary circulation was higher in patients compared with control subjects: 519 ng/min (IQR, 198 to 1276 ng/min) versus -529 ng/min (IQR, -872 to -79 ng/min; P=0.001) and correlated with percent atheroma volume (rs=0.37, P=0.04). Net production of lysophosphatidylcholine was higher in patients compared with control subjects: 199 ng/min (IQR, -592 to 470 ng/min) versus -505 ng/min (IQR, -1119 to 0 ng/min; P=0.03) and correlated with coronary endothelial dysfunction (rs=0.5, P=0.005). C-reactive protein was not significantly different between the groups. CONCLUSIONS - Early coronary atherosclerosis in humans is characterized by local production of Lp-PLA2. Local coronary production of lysophosphatidylcholine, the active product of Lp-PLA2, is associated with endothelial dysfunction. These results support the role for Lp-PLA2 in the mechanism of regional vascular inflammation and atherosclerosis in humans.

AB - BACKGROUND - Lipoprotein-associated phospholipase A2 (Lp-PLA2) is a novel marker and participant in vascular inflammation. Inflammation also is associated with coronary atherosclerosis. We tested the hypothesis that local coronary production of Lp-PLA2 is enhanced in patients with early coronary atherosclerosis and associated with local endothelial function. METHODS AND RESULTS - Coronary angiography, blood flow, flow reserve, endothelial function assessment, and intravascular ultrasound with volumetric analysis were performed in 15 patients with mild coronary atherosclerosis and in 15 control subjects. Plasma samples were collected simultaneously from the left main coronary artery and coronary sinus for measurement of Lp-PLA2, lysophosphatidylcholine (a product of Lp-PLA2), and C-reactive protein. Hemodynamic parameters and cholesterol were similar in both groups. Arterial Lp-PLA2 levels were similar in patients and control subjects: 225 ng/mL (interquartile range [IQR], 196 to 273 ng/mL) versus 221 ng/mL (IQR, 177 to 294 ng/mL). Lp-PLA2 net production in the coronary circulation was higher in patients compared with control subjects: 519 ng/min (IQR, 198 to 1276 ng/min) versus -529 ng/min (IQR, -872 to -79 ng/min; P=0.001) and correlated with percent atheroma volume (rs=0.37, P=0.04). Net production of lysophosphatidylcholine was higher in patients compared with control subjects: 199 ng/min (IQR, -592 to 470 ng/min) versus -505 ng/min (IQR, -1119 to 0 ng/min; P=0.03) and correlated with coronary endothelial dysfunction (rs=0.5, P=0.005). C-reactive protein was not significantly different between the groups. CONCLUSIONS - Early coronary atherosclerosis in humans is characterized by local production of Lp-PLA2. Local coronary production of lysophosphatidylcholine, the active product of Lp-PLA2, is associated with endothelial dysfunction. These results support the role for Lp-PLA2 in the mechanism of regional vascular inflammation and atherosclerosis in humans.

KW - Atherosclerosis

KW - Endothelium

KW - Inflammation

KW - Vasculature

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