Local control of oligodendrocyte development in isolated dorsal mouse spinal cord

Caroline R. Sussman, Kimberly L. Dyer, Mark Marchionni, Robert H. Miller

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The earliest oligodendrocyte precursors have been proposed to arise in the ventral ventricular zone of the embryonic thoraco-lumbar spinal cord and subsequently migrate to populate dorsal spinal cord. Using the expression of O4 immunoreactivity to define cells of the oligodendrocyte lineage, the development of oligodendrocytes in different regions of the mouse spinal cord was assayed. Consistent with earlier studies in other species, isolated explants of E11 ventral but not dorsal mouse spinal cord developed oligodendrocytes after 7 days in vitro. In contrast, in cultures derived from E13 embryos O4+ oligodendrocytes developed in both ventral and dorsal cultures after 5 days in vitro. These data are consistent with a ventral to dorsal migration of committed oligodendrocyte progenitors occurring between E11 and E13. Although isolated early embryonic dorsal spinal cord does not give rise to oligodendrocytes in short term cultures, in long term cultures O4+ cells develop in a subset of dorsal explants. After 10 days in vitro approximately 25% of both cervical and thoraco-lumbar E11 derived dorsal explants contained significant numbers of O4+ cells. The molecular requirements for the dorsally-derived oligodendrocytes was similar to that in ventral cord. The appearance of O4+ cells was dependent on sonic hedgehog and enhanced by neuregulin. These data suggest that early embryonic dorsal mouse spinal cord has an independent potential to generate oligodendrocytes under appropriate conditions. Whether this potential is realized during normal spinal cord development is currently unknown. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)413-420
Number of pages8
JournalJournal of Neuroscience Research
Volume59
Issue number3
DOIs
StatePublished - Feb 1 2000
Externally publishedYes

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Oligodendroglia
Spinal Cord
Neuregulins
Hedgehogs
Cell Lineage
Embryonic Structures
Cell Culture Techniques
Cell Count

Keywords

  • Cell differentiation
  • Central nervous system
  • Mouse embryogenesis
  • Neuregulins
  • Sonic hedgehog

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Local control of oligodendrocyte development in isolated dorsal mouse spinal cord. / Sussman, Caroline R.; Dyer, Kimberly L.; Marchionni, Mark; Miller, Robert H.

In: Journal of Neuroscience Research, Vol. 59, No. 3, 01.02.2000, p. 413-420.

Research output: Contribution to journalArticle

Sussman, Caroline R. ; Dyer, Kimberly L. ; Marchionni, Mark ; Miller, Robert H. / Local control of oligodendrocyte development in isolated dorsal mouse spinal cord. In: Journal of Neuroscience Research. 2000 ; Vol. 59, No. 3. pp. 413-420.
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