LMPCR for detection of oligonucleotide-directed triple helix formation: A cautionary note

Nicole A. Becker, L. James Maher

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We note that precautions are necessary when ligation-mediated PCR (LMPCR) is applied to the detection of oligonucleotide-directed triple helix formation in vitro and in vivo. Synthetic oligonucleotides applied to cell cultures can persist after chemical treatment and genomic DNA isolation and inhibit a kg step in LMPCR, causing an artifact that simulates a triplex footprint. Residual oligonucleotides apparently form triplexes during LMPCR, blocking ligation of the unidirectional linker in a site-specific manner. We show that careful removal of residual oligonucleotide prior to LMPCR alleviates this problem.

Original languageEnglish (US)
Pages (from-to)313-316
Number of pages4
JournalAntisense and Nucleic Acid Drug Development
Volume9
Issue number3
DOIs
StatePublished - Jan 1 1999

ASJC Scopus subject areas

  • Genetics
  • Pharmacology

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