Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes

Zhongying Wang; Tatyana Gurlo; Aleksey V. Matveyenko; David Elashoff; Peiyu Wang; Madeline Rosenberger; Jason A. Junge; Raymond C. Stevens; Kate L. White; Scott E. Fraser; Peter C. Butler

doi:10.1038/s42003-021-02113-1

Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes

Zhongying Wang, Tatyana Gurlo, Aleksey V. Matveyenko, David Elashoff, Peiyu Wang, Madeline Rosenberger, Jason A. Junge, Raymond C. Stevens, Kate L. White, Scott E. Fraser, Peter C. Butler

Physiology & Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Type 2 diabetes is characterized by β and α cell dysfunction. We used phasor-FLIM (Fluorescence Lifetime Imaging Microscopy) to monitor oxidative phosphorylation and glycolysis in living islet cells before and after glucose stimulation. In healthy cells, glucose enhanced oxidative phosphorylation in β cells and suppressed oxidative phosphorylation in α cells. In Type 2 diabetes, glucose increased glycolysis in β cells, and only partially suppressed oxidative phosphorylation in α cells. FLIM uncovers key perturbations in glucose induced metabolism in living islet cells and provides a sensitive tool for drug discovery in diabetes.

Original language	English (US)
Article number	594
Journal	Communications Biology
Volume	4
Issue number	1
DOIs	https://doi.org/10.1038/s42003-021-02113-1
State	Published - Dec 2021

ASJC Scopus subject areas

Medicine (miscellaneous)
General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences

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10.1038/s42003-021-02113-1

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title = "Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes",

abstract = "Type 2 diabetes is characterized by β and α cell dysfunction. We used phasor-FLIM (Fluorescence Lifetime Imaging Microscopy) to monitor oxidative phosphorylation and glycolysis in living islet cells before and after glucose stimulation. In healthy cells, glucose enhanced oxidative phosphorylation in β cells and suppressed oxidative phosphorylation in α cells. In Type 2 diabetes, glucose increased glycolysis in β cells, and only partially suppressed oxidative phosphorylation in α cells. FLIM uncovers key perturbations in glucose induced metabolism in living islet cells and provides a sensitive tool for drug discovery in diabetes.",

author = "Zhongying Wang and Tatyana Gurlo and Matveyenko, {Aleksey V.} and David Elashoff and Peiyu Wang and Madeline Rosenberger and Junge, {Jason A.} and Stevens, {Raymond C.} and White, {Kate L.} and Fraser, {Scott E.} and Butler, {Peter C.}",

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doi = "10.1038/s42003-021-02113-1",

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AU - Wang, Zhongying

AU - Gurlo, Tatyana

AU - Matveyenko, Aleksey V.

AU - Elashoff, David

AU - Wang, Peiyu

AU - Rosenberger, Madeline

AU - Junge, Jason A.

AU - Stevens, Raymond C.

AU - White, Kate L.

AU - Fraser, Scott E.

AU - Butler, Peter C.

PY - 2021/12

Y1 - 2021/12

N2 - Type 2 diabetes is characterized by β and α cell dysfunction. We used phasor-FLIM (Fluorescence Lifetime Imaging Microscopy) to monitor oxidative phosphorylation and glycolysis in living islet cells before and after glucose stimulation. In healthy cells, glucose enhanced oxidative phosphorylation in β cells and suppressed oxidative phosphorylation in α cells. In Type 2 diabetes, glucose increased glycolysis in β cells, and only partially suppressed oxidative phosphorylation in α cells. FLIM uncovers key perturbations in glucose induced metabolism in living islet cells and provides a sensitive tool for drug discovery in diabetes.

AB - Type 2 diabetes is characterized by β and α cell dysfunction. We used phasor-FLIM (Fluorescence Lifetime Imaging Microscopy) to monitor oxidative phosphorylation and glycolysis in living islet cells before and after glucose stimulation. In healthy cells, glucose enhanced oxidative phosphorylation in β cells and suppressed oxidative phosphorylation in α cells. In Type 2 diabetes, glucose increased glycolysis in β cells, and only partially suppressed oxidative phosphorylation in α cells. FLIM uncovers key perturbations in glucose induced metabolism in living islet cells and provides a sensitive tool for drug discovery in diabetes.

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Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes

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ASJC Scopus subject areas

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