Liposome-enhanced MRI of neointimal lesions in the ApoE-KO mouse

Willem J.M. Mulder, Kim Douma, Gerben A. Koning, Marc A. Van Zandvoort, Esther Lutgens, Mat J. Daemen, Klaas Nicolay, Gustav J. Strijkers

Research output: Contribution to journalArticlepeer-review

Abstract

Conventional high-resolution MRI is capable of detecting lipid-rich atherosclerotic plaques in both human atherosclerosis and animal models of atherosclerosis. In this study we induced neointimal lesions in ApoE-KO mice by placing a constrictive collar around the right carotid artery. The model was imaged with conventional multispectral MRI, and the thickened wall could not be distinguished from surrounding tissue. We then tested paramagnetic liposomes (mean size = 90 nm) for their ability to improve MRI visualization of induced thickening, using Gd-DTPA as a control. T1-weighted (T 1-W), black-blood MRI of the neck area of the mice was performed before and 15 min, 45 min, and 24 hr after intravenous injection of either paramagnetic liposomes or Gd-DTPA. The collared vessel wall of mice that were injected with liposomes showed a pronounced signal enhancement of ∼100% immediately after injection, which was sustained largely until 24 hr postinjection. In contrast, the vessel wall of all controls (left carotid artery and animals injected with Gd-DTPA) did not show significant contrast enhancement at those time points. This study demonstrates that intimal thickening in ApoE-KO mice can be effectively detected by contrast-enhanced (CE)-MRI upon injection of paramagnetic liposomes.

Original languageEnglish (US)
Pages (from-to)1170-1174
Number of pages5
JournalMagnetic Resonance in Medicine
Volume55
Issue number5
DOIs
StatePublished - May 2006

Keywords

  • ApoE-KO
  • Atherosclerosis
  • Contrast agent
  • Liposome
  • Molecular MRI
  • Mouse

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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