Lipopolysaccharides with Acylation Defects Potentiate TLR4 Signaling and Shape T Cell Responses

Anna Martirosyan, Yoichiro Ohne, Clara Degos, Laurent Gorvel, Ignacio Moriyón, Sangkon Oh, Jean Pierre Gorvel

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Lipopolysaccharides or endotoxins are components of Gram-negative enterobacteria that cause septic shock in mammals. However, a LPS carrying hexa-acyl lipid A moieties is highly endotoxic compared to a tetra-acyl LPS and the latter has been considered as an antagonist of hexa-acyl LPS-mediated TLR4 signaling. We investigated the relationship between the structure and the function of bacterial LPS in the context of human and mouse dendritic cell activation. Strikingly, LPS with acylation defects were capable of triggering a strong and early TLR4-dependent DC activation, which in turn led to the activation of the proteasome machinery dampening the pro-inflammatory cytokine secretion. Upon activation with tetra-acyl LPS both mouse and human dendritic cells triggered CD4+ T and CD8+ T cell responses and, importantly, human myeloid dendritic cells favored the induction of regulatory T cells. Altogether, our data suggest that LPS acylation controlled by pathogenic bacteria might be an important strategy to subvert adaptive immunity.

Original languageEnglish (US)
Article numbere55117
JournalPloS one
Volume8
Issue number2
DOIs
StatePublished - Feb 4 2013

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Martirosyan, A., Ohne, Y., Degos, C., Gorvel, L., Moriyón, I., Oh, S., & Gorvel, J. P. (2013). Lipopolysaccharides with Acylation Defects Potentiate TLR4 Signaling and Shape T Cell Responses. PloS one, 8(2), [e55117]. https://doi.org/10.1371/journal.pone.0055117