Lipopolysaccharide-induced cyclooxygenase-2 expression in mouse transformed clara cells

Rodney Jr. Britt, Morgan L. Locy, Trent E. Tipple, Leif D. Nelin, Lynette K. Rogers

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background/Aims: Exacerbation of innate immune responses can contribute to development of acute lung injury. Multiple cell populations, including the bronchiolar epithelium, coordinate these inflammatory responses. Clara cells, non-ciliated epithelial cells, are located in the distal airways in humans and conducting airways in mice. These cells actively participate in innate immune responses but their precise contributions remain poorly defined. Methods: To test the hypothesis that E. coli lipopolysaccaride (LPS) treatment stimulates production of pro-inflammatory mediators in mouse transformed Clara cells (MTCC), MTCC were treated with E. coli lipopolysaccaride (LPS). Results: LPS increased COX-2 expression and stimulated production of prostaglandins, including prostaglandin E 2 (PGE 2). Enhanced mitogen activated protein kinase (MAPK) activation, nuclear factor-κB (NFκB) activation, and chemokine production were observed in MTCC in response to LPS treatment. Conclusions: While the role for Clara cells in the regulation of host defense and the progression of acute lung injury needs further characterization, our data suggests the importance of this unique cell population in the pathogenesis of LPS-induced acute lung injury.

Original languageEnglish (US)
Pages (from-to)213-222
Number of pages10
JournalCellular Physiology and Biochemistry
Volume29
Issue number1-2
DOIs
StatePublished - Apr 23 2012
Externally publishedYes

Fingerprint

Cyclooxygenase 2
Lipopolysaccharides
Acute Lung Injury
Innate Immunity
Escherichia coli
Prostaglandins E
Mitogen-Activated Protein Kinases
Chemokines
Population
Prostaglandins
Epithelium
Epithelial Cells

Keywords

  • Chemotaxis
  • Clara cells
  • COX-2
  • Inflammation
  • LPS
  • Prostaglandins

ASJC Scopus subject areas

  • Physiology

Cite this

Lipopolysaccharide-induced cyclooxygenase-2 expression in mouse transformed clara cells. / Britt, Rodney Jr.; Locy, Morgan L.; Tipple, Trent E.; Nelin, Leif D.; Rogers, Lynette K.

In: Cellular Physiology and Biochemistry, Vol. 29, No. 1-2, 23.04.2012, p. 213-222.

Research output: Contribution to journalArticle

Britt, Rodney Jr. ; Locy, Morgan L. ; Tipple, Trent E. ; Nelin, Leif D. ; Rogers, Lynette K. / Lipopolysaccharide-induced cyclooxygenase-2 expression in mouse transformed clara cells. In: Cellular Physiology and Biochemistry. 2012 ; Vol. 29, No. 1-2. pp. 213-222.
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