Lipopolysaccharide and hypoxia/ischemia induced IL-2 expression by microglia in neonatal brain

Sylvie Girard, Annie Larouche, Hazim Kadhim, Marek Rola-Pleszczynski, Fernand Gobeil, Guillaume Sébire

Research output: Contribution to journalArticlepeer-review

Abstract

Using a model of perinatal brain lesions induced by lipopolysaccharide and hypoxia/ischemia, we hypothesized that interleukin-2 (IL-2), a neurotoxic cytokine, was enhanced within injured brains. We showed that lipopolysaccharide and hypoxia/ischemia enhanced both intracerebral IL-2 mRNA and protein levels, with a maximum increase upon lipopolysaccharide and hypoxia/ischemia. The lack of detectable T lymphocytes suggested the synthesis of IL-2 by neural cells. Lipopolysaccharide and hypoxia triggered IL-2 synthesis by cultured microglia with a peak after exposure to lipopolysaccharide and hypoxia. Double-labeling showed, in vivo and in vitro, that IL-2 immunoreactivity was colocalized with a microglia/macrophage marker. These results disclosed the ability of microglia to produce IL-2 and also suggest the implication of IL-2 in neural cell death triggered by perinatal lipopolysaccharide and hypoxia/ischemia exposures.

Original languageEnglish (US)
Pages (from-to)997-1002
Number of pages6
JournalNeuroReport
Volume19
Issue number10
DOIs
StatePublished - Jul 2 2008

Keywords

  • Cerebral palsy
  • Hypoxia/ischemia
  • Interleukin-2
  • Lipopolysaccharide
  • Perinatal brain

ASJC Scopus subject areas

  • Neuroscience(all)

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