Lipolysis of visceral adipocyte triglyceride by pancreatic lipases converts mild acute pancreatitis to severe pancreatitis independent of necrosis and inflammation

Krutika Patel, Ram N. Trivedi, Chandra Durgampudi, Pawan Noel, Rachel A. Cline, James P. Delany, Sarah Navina, Vijay Prem Singh

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Visceral fat necrosis has been associated with severe acute pancreatitis (SAP) for over 100 years; however, its pathogenesis and role in SAP outcomes are poorly understood. Based on recent work suggesting that pancreatic fat lipolysis plays an important role in SAP, we evaluated the role of pancreatic lipases in SAP-associated visceral fat necrosis, the inflammatory response, local injury, and outcomes of acute pancreatitis (AP). For this, cerulein pancreatitis was induced in lean and obese mice, alone or with the lipase inhibitor orlistat and parameters of AP induction (serum amylase and lipase), fat necrosis, pancreatic necrosis, and multisystem organ failure, and inflammatory response were assessed. Pancreatic lipases were measured in fat necrosis and were overexpressed in 3T3-L1 cells. We noted obesity to convert mild cerulein AP to SAP with greater cytokines, unsaturated fatty acids (UFAs), and multisystem organ failure, and 100% mortality without affecting AP induction or pancreatic necrosis. Increased pancreatic lipase amounts and activity were noted in the extensive visceral fat necrosis of dying obese mice. Lipase inhibition reduced fat necrosis, UFAs, organ failure, and mortality but not the parameters of AP induction. Pancreatic lipase expression increased lipolysis in 3T3-L1 cells. We conclude that UFAs generated via lipolysis of visceral fat by pancreatic lipases convert mild AP to SAP independent of pancreatic necrosis and the inflammatory response.

Original languageEnglish (US)
Pages (from-to)808-819
Number of pages12
JournalAmerican Journal of Pathology
Volume185
Issue number3
DOIs
StatePublished - Mar 1 2015

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Lipolysis
Lipase
Adipocytes
Pancreatitis
Necrosis
Inflammation
Fat Necrosis
Intra-Abdominal Fat
Unsaturated Fatty Acids
3T3-L1 Cells
Ceruletide
Obese Mice
Mortality
Amylases
Obesity
Fats

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Lipolysis of visceral adipocyte triglyceride by pancreatic lipases converts mild acute pancreatitis to severe pancreatitis independent of necrosis and inflammation. / Patel, Krutika; Trivedi, Ram N.; Durgampudi, Chandra; Noel, Pawan; Cline, Rachel A.; Delany, James P.; Navina, Sarah; Singh, Vijay Prem.

In: American Journal of Pathology, Vol. 185, No. 3, 01.03.2015, p. 808-819.

Research output: Contribution to journalArticle

Patel, Krutika ; Trivedi, Ram N. ; Durgampudi, Chandra ; Noel, Pawan ; Cline, Rachel A. ; Delany, James P. ; Navina, Sarah ; Singh, Vijay Prem. / Lipolysis of visceral adipocyte triglyceride by pancreatic lipases converts mild acute pancreatitis to severe pancreatitis independent of necrosis and inflammation. In: American Journal of Pathology. 2015 ; Vol. 185, No. 3. pp. 808-819.
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