Lipid organization in peripheral nerve: Natural abundance carbon-13 NMR spectroscopy on intact tissues

Yuri Wedmid, Alfred C. Lais, Peter J. Dyck, Wolfgang J. Baumann

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Native structures of peripheral nerve myelin can directly be observed by carbon-13 nuclear magnetic resonance (NMR) spectroscopy after dissecting epineurial and perineurial tissue sheaths from the endoneurium. NMR spectra of the intact endoneurial tissue describe a highly ordered and largely immobilized lipid phase in the myelin membrane which is in marked contrast to the fluid-like lipid distribution in the intracellular lipid droplets of epineurium and perineurium. Membrane reconstitution experiments with total endoneurial lipids, but without protein, suggest a primary role of the lipids in myelin bilayer assembly. Reconstitution experiments also indicate that polar lipid-protein interactions in native myelin involve the CH2-N function of choline phospholipids, in particular.

Original languageEnglish (US)
Pages (from-to)139-147
Number of pages9
JournalTopics in Catalysis
Volume97
Issue number1
DOIs
StatePublished - Jan 1 1980

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ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Biophysics
  • Molecular Biology

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