Linking expression of FOXM1, CEP55 and HELLS to tumorigenesis in oropharyngeal squamous cell carcinoma

Jeffrey R. Janus, Rebecca R. Laborde, Alexandra J. Greenberg, Vivian W. Wang, Wei Wei, Anna Trier, Steven M. Olsen, Eric J. Moore, Kerry D. Olsen, David I. Smith

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Objectives/Hypothesis: To investigate the relationship between the expression of FOXM1, CEP55, and HELLS in oropharyngeal squamous cell carcinoma to human papillomavirus (HPV), smoking, and tumor stage. Study Design: Retrospective cohort study. Methods: Transcriptome data were analyzed from matched tumor-normal samples taken from patients with oropharyngeal squamous cell carcinoma. Data were previously generated using deep-sequencing techniques (mRNA-Seq). Transcript levels of all three genes were validated using the NanoString nCounter system in a larger group of patients. Analyses were conducted to assess possible associations between expression levels and HPV infection status, smoking status, or tumor staging. Results: FOXM1, CEP55, and HELLS were all overexpressed in oropharyngeal squamous cell carcinoma tissue when compared to normal tissue. Significant trends were found between expression levels of FOXM1, CEP55, and HELLS and tumor staging. Tumors staged 3 or greater showed significantly higher levels of expression compared with those staged 1. No significant association or trend was found between expression of any genes of interest and etiologic subgroupings (i.e., HPV, smoking). Conclusions: FOXM1, CEP55, and HELLS were all overexpressed in oropharyngeal squamous cell carcinoma. Gene expression is related to tumor stage. The significant association between the expression of these genes and advanced tumor stage suggest that they may play a role in tumorigenesis.

Original languageEnglish (US)
Pages (from-to)2598-2603
Number of pages6
JournalLaryngoscope
Volume121
Issue number12
DOIs
StatePublished - Dec 1 2011

Keywords

  • FOXM1
  • Oropharyngeal cancer
  • tumorigenesis

ASJC Scopus subject areas

  • Otorhinolaryngology

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    Janus, J. R., Laborde, R. R., Greenberg, A. J., Wang, V. W., Wei, W., Trier, A., Olsen, S. M., Moore, E. J., Olsen, K. D., & Smith, D. I. (2011). Linking expression of FOXM1, CEP55 and HELLS to tumorigenesis in oropharyngeal squamous cell carcinoma. Laryngoscope, 121(12), 2598-2603. https://doi.org/10.1002/lary.22379