Lines of Murine Oligodendroglial Precursor Cells Immortalized by an Activated neu Tyrosine Kinase Show Distinct Degrees of Interaction with Axons In Vitro and In Vivo

M. Jung, E. Krämer, M. Grzenkowski, K. Tang, W. Blakemore, A. Aguzzi, K. Khazaie, K. Chlichlia, G. von Blankenfeld, H. Kettenmann, J. Trotter

Research output: Contribution to journalArticle

184 Scopus citations

Abstract

Replication‐defective retroviruses expressing the t‐neu oncogene, or a hybrid protein with the neu tyrosine kinase linked to the external region of the human epidermal growth factor receptor (egfr‐neu), were used to establish lines of murine oligodendroglial precursor cells. Differentiation of the t‐neu lines into myelin‐associated glycoprotein (MAG)‐positive oligodendrocytes was induced by dibutyryl cAMP, and the egfr‐neu line showed limited differentiation in vitro upon withdrawal of epidermal growth factor. Cerebellar granule cell neurons expressed mitogens for the cell lines. Upon transplantation into demyelinated lesions, t‐neu line cells engaged with the demyelinated axons whereas the egfr‐neu line cells differentiated further and ensheathed the axons. These cell lines thus interact with neurons in vitro and in vivo and can be used as tools to define the molecules involved in different stages of neuron‐glia interaction.

Original languageEnglish (US)
Pages (from-to)1245-1265
Number of pages21
JournalEuropean Journal of Neuroscience
Volume7
Issue number6
DOIs
StatePublished - Jun 1995

Keywords

  • immortalization
  • myelin‐associated glycoprotein
  • neu tyrosine kinase
  • oligodendrocyte‐neuron interaction
  • retroviral vector

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Lines of Murine Oligodendroglial Precursor Cells Immortalized by an Activated neu Tyrosine Kinase Show Distinct Degrees of Interaction with Axons In Vitro and In Vivo'. Together they form a unique fingerprint.

  • Cite this