Lessons from morpholino-based screening in zebrafish

Victoria M. Bedell, Stephanie E. Westcot, Stephen C. Ekker

Research output: Contribution to journalArticle

95 Scopus citations

Abstract

Morpholino oligonucleotides (MOs) are an effective, gene-specific antisense knockdown technology used in many model systems. Here we describe the application of MOs in zebrafish (Danio rerio) for in vivo functional characterization of gene activity. We summarize our screening experience beginning with gene target selection. We then discuss screening parameter considerations and data and database management. Finally, we emphasize the importance of off-target effect management and thorough downstream phenotypic validation. We discuss current morpholino limitations, including reduced stability when stored in aqueous solution. Advances in MO technology now provide a measure of spatiotemporal control over MO activity, presenting the opportunity for incorporating more finely tuned analyses into MO-based screening. Therefore, with careful management, MOs remain a valuable tool for discovery screening as well as individual gene knockdown analysis.

Original languageEnglish (US)
Article numberelr021
Pages (from-to)181-188
Number of pages8
JournalBriefings in Functional Genomics
Volume10
Issue number4
DOIs
StatePublished - Jul 2011

Keywords

  • Knockdown
  • Morpholinos
  • Zebrafish

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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