Leptin upregulates caveolin-1 expression: Implications for development of atherosclerosis

Prachi Singh, Timothy E. Peterson, Fatima H. Sert-Kuniyoshi, Michael D. Jensen, Virend K. Somers

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Objective: To determine the role of hyperleptinemia on caveolin-1 expression and leptin signaling. Methods: Endothelial cells are critical to atherosclerosis development; therefore we investigated hyperleptinemia in cultured vascular endothelial cells. Dose-dependent effect of leptin on caveolin-1 expression was determined by Western blot analysis. Also, the consequence of increased caveolin-1 expression on leptin signaling was investigated by adenovirus mediated caveolin-1 overexpression. The effect of increased caveolin-1 expression on leptin-dependent activation of ERK1/2 and eNOS was determined by Western blot analysis. Results: Leptin upregulates caveolin-1 protein expression in a dose dependent manner and increased caveolin-1 expression impairs leptin signaling. Conclusions: Leptin increases caveolin-1 protein expression which impairs leptin signaling in vascular endothelial cells. Our study identifies an additional leptin mediated proatherogenic mechanism and a novel caveolin-1 dependent leptin feedback mechanism which may have implications for development of peripheral leptin resistance in the endothelium.

Original languageEnglish (US)
Pages (from-to)499-502
Number of pages4
JournalAtherosclerosis
Volume217
Issue number2
DOIs
StatePublished - Aug 2011

Keywords

  • Atherosclerosis
  • Caveolin-1
  • Cell signaling
  • Leptin
  • Obesity

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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