TY - JOUR
T1 - Leptin receptor immunoreactivity in sympathetic prevertebral ganglion neurons of mouse and rat
AU - Miller, Steven M.
AU - Schmalz, Philip F.
AU - Benarroch, Eduardo E.
AU - Szurszewski, Joseph H.
N1 - Funding Information:
This work was supported by NIH Grant DK 17632. The authors express appreciation to Jan Applequist and James D. Schmelzer for their assistance and Dr. Simon Gibbons for his suggestions.
PY - 1999/4/16
Y1 - 1999/4/16
N2 - Prevertebral ganglia, comprising the inferior mesenteric, superior mesenteric and celiac ganglia, contain the cell bodies of sympathetic neurons that regulate gastrointestinal motility, visceral blood flow, secretion and absorption. In the present study, we report the presence of leptin receptor- like immunoreactivity in the superior mesenteric and celiac ganglia of mice and rats. Ganglion neurons were immunopositive for leptin receptor. Confocal microscopy and three-dimensional (3-D) reconstruction of ganglion neurons filled with Lucifer yellow and then immunostained for leptin receptor showed that leptin receptor immunoreactivity was intracellular and that it was present on structures encircling the nucleus. These results raise the possibility that leptin may affect gastrointestinal function by acting on leptin receptors located in prevertebral ganglion neurons.
AB - Prevertebral ganglia, comprising the inferior mesenteric, superior mesenteric and celiac ganglia, contain the cell bodies of sympathetic neurons that regulate gastrointestinal motility, visceral blood flow, secretion and absorption. In the present study, we report the presence of leptin receptor- like immunoreactivity in the superior mesenteric and celiac ganglia of mice and rats. Ganglion neurons were immunopositive for leptin receptor. Confocal microscopy and three-dimensional (3-D) reconstruction of ganglion neurons filled with Lucifer yellow and then immunostained for leptin receptor showed that leptin receptor immunoreactivity was intracellular and that it was present on structures encircling the nucleus. These results raise the possibility that leptin may affect gastrointestinal function by acting on leptin receptors located in prevertebral ganglion neurons.
KW - Immunohistochemistry
KW - Leptin receptors
KW - Obesity
KW - Prevertebral ganglia
KW - Sympathetic nervous system
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U2 - 10.1016/S0304-3940(99)00215-3
DO - 10.1016/S0304-3940(99)00215-3
M3 - Article
C2 - 10327172
AN - SCOPUS:0033574670
SN - 0304-3940
VL - 265
SP - 75
EP - 78
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 2
ER -