TY - JOUR
T1 - Leptin modulates fast synaptic transmission in dog pancreatic ganglia
AU - Sha, Lei
AU - Szurszewski, Joseph H.
N1 - Funding Information:
This work is supported by NIH Grant DK 17632 from the National Institutes of Health. The authors express their appreciation to Dr. Steven M. Miller for his many helpful comments and suggestions and to Jan Applequist for her secretarial assistance.
PY - 1999/3/26
Y1 - 1999/3/26
N2 - The aim of this study was to determine whether leptin modulates neuronal activity in intrapancreatic ganglion neurons. Intracellular recordings were made in dog pancreatic neurons. Recombinant mouse leptin (313 nM) was added by superfusion. When leptin was present, fast EPSPs which were subthreshold in normal Krebs solution reached threshold for firing action potentials. However, leptin had no significant (P > 0.05, n = 18) effect on either the resting membrane potential or on membrane input resistance. To determine whether leptin increased the postsynaptic sensitivity to acetylcholine, the response was tested by pressure ejection of acetylcholine. Acetylcholine evoked a 9.4 ± 2.2 mV (mean ± SEM, n=5) depolarization in normal Krebs solution. In the presence of leptin, the response was not significantly different (9.6 ± 2.4 mV, P > 0.05). The results suggest that leptin modulates fast synaptic transmission in pancreatic ganglion neurons by acting on presynaptic nerve terminals.
AB - The aim of this study was to determine whether leptin modulates neuronal activity in intrapancreatic ganglion neurons. Intracellular recordings were made in dog pancreatic neurons. Recombinant mouse leptin (313 nM) was added by superfusion. When leptin was present, fast EPSPs which were subthreshold in normal Krebs solution reached threshold for firing action potentials. However, leptin had no significant (P > 0.05, n = 18) effect on either the resting membrane potential or on membrane input resistance. To determine whether leptin increased the postsynaptic sensitivity to acetylcholine, the response was tested by pressure ejection of acetylcholine. Acetylcholine evoked a 9.4 ± 2.2 mV (mean ± SEM, n=5) depolarization in normal Krebs solution. In the presence of leptin, the response was not significantly different (9.6 ± 2.4 mV, P > 0.05). The results suggest that leptin modulates fast synaptic transmission in pancreatic ganglion neurons by acting on presynaptic nerve terminals.
KW - Fast epsp
KW - Intrapancreatic neuron
KW - Leptin
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U2 - 10.1016/S0304-3940(99)00122-6
DO - 10.1016/S0304-3940(99)00122-6
M3 - Article
C2 - 10213143
AN - SCOPUS:0033605709
SN - 0304-3940
VL - 263
SP - 93
EP - 96
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 2-3
ER -