LC-MS-based serum metabolomic analysis reveals dysregulation of phosphatidylcholines in esophageal squamous cell carcinoma

Sartaj Ahmad Mir, Pavithra Rajagopalan, Ankit P. Jain, Aafaque Ahmad Khan, Keshava K. Datta, Sonali V. Mohan, Syed Salman Lateef, Nandini Sahasrabuddhe, B. L. Somani, T. S. Keshava Prasad, Aditi Chatterjee, K. V. Veerendra Kumar, M. VijayaKumar, Rekha V. Kumar, Seetaramanjaneyulu Gundimeda, Akhilesh Pandey, Harsha Gowda

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive cancers with poor prognosis. Here, we carried out liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS)-based untargeted metabolomic analysis of ESCC serum samples. Statistical analysis resulted in the identification of 652 significantly dysregulated molecular features in serum from ESCC patients as compared to the healthy subjects. Phosphatidylcholines were identified as a major class of dysregulated metabolites in this study suggesting potential perturbation of phosphocholine metabolism in ESCC. By using a targeted MS/MS approach both in positive and negative mode, we were able to characterize and confirm the structure of seven metabolites. Our study describes a quantitative LC-MS approach for characterizing dysregulated lipid metabolism in ESCC. Biological significance: Altered metabolism is a hallmark of cancer. We carried out (LC-MS)-based untargeted metabolomic profiling of serum from esophageal squamous cell carcinoma (ESCC) patients to characterize dysregulated metabolites. Phosphatidylcholine metabolism was found to be significantly altered in ESCC. Our study illustrates the use of mass spectrometry-based metabolomic analysis to characterize molecular alterations associated with ESCC. This article is part of a Special Issue entitled: Proteomics in India.

Original languageEnglish (US)
Pages (from-to)96-102
Number of pages7
JournalJournal of Proteomics
Volume127
DOIs
StatePublished - Sep 8 2015
Externally publishedYes

Fingerprint

Metabolomics
Phosphatidylcholines
Serum
Metabolites
Metabolism
Mass spectrometry
Mass Spectrometry
Phosphorylcholine
Esophageal Squamous Cell Carcinoma
Epithelial Cells
Liquid chromatography
Lipid Metabolism
Liquid Chromatography
Proteomics
India
Neoplasms
Statistical methods
Healthy Volunteers

Keywords

  • Cancer
  • Cholines
  • Lyso PCs
  • Metabolism
  • PCs
  • Serum metabolome

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Cite this

LC-MS-based serum metabolomic analysis reveals dysregulation of phosphatidylcholines in esophageal squamous cell carcinoma. / Mir, Sartaj Ahmad; Rajagopalan, Pavithra; Jain, Ankit P.; Khan, Aafaque Ahmad; Datta, Keshava K.; Mohan, Sonali V.; Lateef, Syed Salman; Sahasrabuddhe, Nandini; Somani, B. L.; Keshava Prasad, T. S.; Chatterjee, Aditi; Veerendra Kumar, K. V.; VijayaKumar, M.; Kumar, Rekha V.; Gundimeda, Seetaramanjaneyulu; Pandey, Akhilesh; Gowda, Harsha.

In: Journal of Proteomics, Vol. 127, 08.09.2015, p. 96-102.

Research output: Contribution to journalArticle

Mir, SA, Rajagopalan, P, Jain, AP, Khan, AA, Datta, KK, Mohan, SV, Lateef, SS, Sahasrabuddhe, N, Somani, BL, Keshava Prasad, TS, Chatterjee, A, Veerendra Kumar, KV, VijayaKumar, M, Kumar, RV, Gundimeda, S, Pandey, A & Gowda, H 2015, 'LC-MS-based serum metabolomic analysis reveals dysregulation of phosphatidylcholines in esophageal squamous cell carcinoma', Journal of Proteomics, vol. 127, pp. 96-102. https://doi.org/10.1016/j.jprot.2015.05.013
Mir, Sartaj Ahmad ; Rajagopalan, Pavithra ; Jain, Ankit P. ; Khan, Aafaque Ahmad ; Datta, Keshava K. ; Mohan, Sonali V. ; Lateef, Syed Salman ; Sahasrabuddhe, Nandini ; Somani, B. L. ; Keshava Prasad, T. S. ; Chatterjee, Aditi ; Veerendra Kumar, K. V. ; VijayaKumar, M. ; Kumar, Rekha V. ; Gundimeda, Seetaramanjaneyulu ; Pandey, Akhilesh ; Gowda, Harsha. / LC-MS-based serum metabolomic analysis reveals dysregulation of phosphatidylcholines in esophageal squamous cell carcinoma. In: Journal of Proteomics. 2015 ; Vol. 127. pp. 96-102.
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AU - Jain, Ankit P.

AU - Khan, Aafaque Ahmad

AU - Datta, Keshava K.

AU - Mohan, Sonali V.

AU - Lateef, Syed Salman

AU - Sahasrabuddhe, Nandini

AU - Somani, B. L.

AU - Keshava Prasad, T. S.

AU - Chatterjee, Aditi

AU - Veerendra Kumar, K. V.

AU - VijayaKumar, M.

AU - Kumar, Rekha V.

AU - Gundimeda, Seetaramanjaneyulu

AU - Pandey, Akhilesh

AU - Gowda, Harsha

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