Lateral line, nervous system, and maternal expression of Frizzled 7a during zebrafish embryogenesis

Saulius Sumanas, Hyon J. Kim, Spencer B. Hermanson, Stephen C Ekker

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have isolated and mapped a new wnt receptor frizzled family member, zebrafish frizzled 7a. Fz7a and a previously reported zebrafish fz7 (El-Messaoudi and Renucci, 2001) make an orthologous gene pair, however, they display distinct expression patterns. Fz7a shows strong maternal as well as zygotic expression. Fz7a transcript is enriched dorsally starting with the shield stage. At the end of gastrulation, Fz7a is abundantly expressed within anterior neuroectoderm and expressed more weakly within lateral mesoderm. Fz7a is detected during somitogenesis within the central nervous system, somatic and posterior lateral mesoderm. At 24 hpf, fz7a is expressed in migrating lateral line primordium. At 48 hpf, fz7a is detected in the ear, pectoral fin bud, and within neuromasts, which had originated from the lateral line primordium. Radiation hybrid mapping using panel LN54 (Hukriede et al., 1999) places fz7a on linkage group 6, linked to the marker fi11h08 (distance 0.00cR, LOD score 14.1). To prove that fz7 and fz7a are indeed different genes we mapped fz7 as well using the same LN54 panel. Fz7 mapped to linkage group 9 with a LOD of 12.5, 27.31 cR from wnt 10a in between markers IBD2759 and fb50e04.

Original languageEnglish (US)
Pages (from-to)107-111
Number of pages5
JournalMechanisms of Development
Volume115
Issue number1-2
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Lateral Line System
Mesoderm
Zebrafish
Nervous System
Embryonic Development
Wnt Receptors
Mothers
Radiation Hybrid Mapping
Neural Plate
Gastrulation
Genes
Ear
Central Nervous System

Keywords

  • Expression
  • Frizzled
  • Lateral line
  • Maternal
  • Nervous system
  • Neuromast
  • Wnt
  • Zebrafish

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Lateral line, nervous system, and maternal expression of Frizzled 7a during zebrafish embryogenesis. / Sumanas, Saulius; Kim, Hyon J.; Hermanson, Spencer B.; Ekker, Stephen C.

In: Mechanisms of Development, Vol. 115, No. 1-2, 2002, p. 107-111.

Research output: Contribution to journalArticle

Sumanas, Saulius ; Kim, Hyon J. ; Hermanson, Spencer B. ; Ekker, Stephen C. / Lateral line, nervous system, and maternal expression of Frizzled 7a during zebrafish embryogenesis. In: Mechanisms of Development. 2002 ; Vol. 115, No. 1-2. pp. 107-111.
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