Laser microdissection and mass spectrometry-based proteomics aids the diagnosis and typing of renal amyloidosis

Sanjeev Sethi, Julie A. Vrana, Jason D. Theis, Nelson Leung, Anjali Sethi, Samih H. Nasr, Fernando C. Fervenza, Lynn D. Cornell, Mary E. Fidler, Ahmet Dogan

Research output: Contribution to journalArticle

117 Scopus citations

Abstract

Accurate diagnosis and typing of renal amyloidosis is critical for prognosis, genetic counseling, and treatment. Laser microdissection and mass spectrometry are emerging techniques for the analysis and diagnosis of many renal diseases. Here we present the results of laser microdissection and mass spectrometry performed on 127 cases of renal amyloidosis during 2008-2010. We found the following proteins in the amyloid deposits: immunoglobulin light and heavy chains, secondary reactive serum amyloid A protein, leukocyte cell-derived chemotaxin-2, fibrinogen-α chain, transthyretin, apolipoprotein A-I and A-IV, gelsolin, and Β-2 microglobulin. Thus, laser microdissection of affected areas within the kidney followed by mass spectrometry provides a direct test of the composition of the deposit and forms a useful ancillary technique for the accurate diagnosis and typing of renal amyloidosis in a single procedure.

Original languageEnglish (US)
Pages (from-to)226-234
Number of pages9
JournalKidney international
Volume82
Issue number2
DOIs
StatePublished - Jul 2 2012

Keywords

  • amyloid typing
  • amyloidosis
  • laser microdissection
  • mass spectrometry
  • proteomics

ASJC Scopus subject areas

  • Nephrology

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    Sethi, S., Vrana, J. A., Theis, J. D., Leung, N., Sethi, A., Nasr, S. H., Fervenza, F. C., Cornell, L. D., Fidler, M. E., & Dogan, A. (2012). Laser microdissection and mass spectrometry-based proteomics aids the diagnosis and typing of renal amyloidosis. Kidney international, 82(2), 226-234. https://doi.org/10.1038/ki.2012.108