Laser-induced fluorescence in the detection of esophageal carcinoma

Kenneth Ke Ning Wang, Kumar Gutta, Mark A. Laukka, John Densmore

Research output: Chapter in Book/Report/Conference proceedingConference contribution

9 Citations (Scopus)

Abstract

Laser induced fluorescence (LIF) is a technique which can perform an 'optical biopsy' of gastrointestinal mucosa. LIF was performed in resected specimens using a pulsed N 2-laser coupled fiberoptically to a probe. Fluorescence was measured using a 0.2 meter spectroscope with an intensified photodiode array. Measurements were made on fresh (<30 minutes after resection) esophageal specimens containing normal mucosa, Barrett's esophagus, and adenocarcinoma. Each tissue section was examined using an optical probe consisting of a central fiber for delivering the excitation energy and a 6 fiber bundle surrounding the central fiber for detection of the fluorescence. An excitation wavelength of 337 nm was used which generated 3-ns pulses while fluorescence intensities were acquired from 300-800 nm. Spectra were obtained from each section in a standardized fashion and background spectra subtracted. Fluorescence readings were taken from 54 normal esophageal sections and 32 sections of adenocarcinoma. A fluorescence index obtained from the tumor sections was 0.68±0.01 compared with 0.51±0.01 for the normal sections (p<0.001). Using a discriminant value of 0.65, this technique had a sensitivity of 81% and a specificity of 100% for detection of malignant tissue. The positive predictive value was 100% and the negative predictive value was 90% for an overall accuracy of 93%. LIF is a promising technique which has the capability of distinguishing normal versus malignant tissue in the esophagus with good accuracy.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsRinaldo Cubeddu, Renato Marchesini, Serge R. Mordon, Katarina Svanberg, Herbert H. Rinneberg, al et al
Pages14-18
Number of pages5
Volume2324
StatePublished - 1995
EventOptical Biopsy and Fluorescence Spectroscopy and Imaging - Lille, Fr
Duration: Sep 9 1994Sep 10 1994

Other

OtherOptical Biopsy and Fluorescence Spectroscopy and Imaging
CityLille, Fr
Period9/9/949/10/94

Fingerprint

laser induced fluorescence
cancer
Fluorescence
Lasers
fluorescence
esophagus
fibers
Tissue
Fibers
probes
bundles
excitation
photodiodes
Excitation energy
Biopsy
tumors
Photodiodes
spectrometers
Tumors
sensitivity

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Wang, K. K. N., Gutta, K., Laukka, M. A., & Densmore, J. (1995). Laser-induced fluorescence in the detection of esophageal carcinoma. In R. Cubeddu, R. Marchesini, S. R. Mordon, K. Svanberg, H. H. Rinneberg, & A. et al (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 2324, pp. 14-18)

Laser-induced fluorescence in the detection of esophageal carcinoma. / Wang, Kenneth Ke Ning; Gutta, Kumar; Laukka, Mark A.; Densmore, John.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / Rinaldo Cubeddu; Renato Marchesini; Serge R. Mordon; Katarina Svanberg; Herbert H. Rinneberg; al et al. Vol. 2324 1995. p. 14-18.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wang, KKN, Gutta, K, Laukka, MA & Densmore, J 1995, Laser-induced fluorescence in the detection of esophageal carcinoma. in R Cubeddu, R Marchesini, SR Mordon, K Svanberg, HH Rinneberg & A et al (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 2324, pp. 14-18, Optical Biopsy and Fluorescence Spectroscopy and Imaging, Lille, Fr, 9/9/94.
Wang KKN, Gutta K, Laukka MA, Densmore J. Laser-induced fluorescence in the detection of esophageal carcinoma. In Cubeddu R, Marchesini R, Mordon SR, Svanberg K, Rinneberg HH, et al A, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2324. 1995. p. 14-18
Wang, Kenneth Ke Ning ; Gutta, Kumar ; Laukka, Mark A. ; Densmore, John. / Laser-induced fluorescence in the detection of esophageal carcinoma. Proceedings of SPIE - The International Society for Optical Engineering. editor / Rinaldo Cubeddu ; Renato Marchesini ; Serge R. Mordon ; Katarina Svanberg ; Herbert H. Rinneberg ; al et al. Vol. 2324 1995. pp. 14-18
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