TY - JOUR
T1 - Laser imaging for rapid Microbial Source Tracking
AU - Bin, Chen
AU - Hao, Gong
AU - Xu, Zhang
AU - Patankar, Prithviraj P.
AU - Sadowsky, Michael J.
AU - Tseng, Charles C.
PY - 2010
Y1 - 2010
N2 - DNA fingerprinting, PCR and other genomic technologies have recently been used to determine sources of fecal bacteria in waterways. Here, we report on the development of a simple and automated optical method for potential use in Microbial Source Tracking (MST) of E. coli. The method employs laser imaging of bacterial colonies and high-resolution optical scattering image analysis for information extraction and classification. Cross validation is used to statistically evaluate the robustness of the classifiers. The entire image analysis procedure can be fully automated, making this a potentially useful tool for future MST studies.
AB - DNA fingerprinting, PCR and other genomic technologies have recently been used to determine sources of fecal bacteria in waterways. Here, we report on the development of a simple and automated optical method for potential use in Microbial Source Tracking (MST) of E. coli. The method employs laser imaging of bacterial colonies and high-resolution optical scattering image analysis for information extraction and classification. Cross validation is used to statistically evaluate the robustness of the classifiers. The entire image analysis procedure can be fully automated, making this a potentially useful tool for future MST studies.
KW - Image classification
KW - Image decomposition
KW - Laser imaging
KW - MST
KW - Microbial Source Tracking
UR - http://www.scopus.com/inward/record.url?scp=78651381987&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78651381987&partnerID=8YFLogxK
U2 - 10.1504/IJCBDD.2010.038023
DO - 10.1504/IJCBDD.2010.038023
M3 - Article
C2 - 21228453
AN - SCOPUS:78651381987
SN - 1756-0756
VL - 3
SP - 177
EP - 186
JO - International Journal of Computational Biology and Drug Design
JF - International Journal of Computational Biology and Drug Design
IS - 3
ER -