Large-scale production of mature neurons from human pluripotent stem cells in a three-dimensional suspension culture system

Alessandra Rigamonti, Giuliana G. Repetti, Chicheng Sun, Feodor D. Price, Danielle C. Reny, Francesca Rapino, Karen Weisinger, Chen Benkler, Quinn Peterson, Lance S. Davidow, Emil M. Hansson, Lee L. Rubin

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Human pluripotent stem cells (hPSCs) offer a renewable source of cells that can be expanded indefinitely and differentiated into virtually any type of cell in the human body, including neurons. This opens up unprecedented possibilities to study neuronal cell and developmental biology and cellular pathology of the nervous system, provides a platform for the screening of chemical libraries that affect these processes, and offers a potential source of transplantable cells for regenerative approaches to neurological disease. However, defining protocols that permit a large number and high yield of neurons has proved difficult. We present differentiation protocols for the generation of distinct subtypes of neurons in a highly reproducible manner, with minimal experiment-to-experiment variation. These neurons form synapses with neighboring cells, exhibit spontaneous electrical activity, and respond appropriately to depolarization. hPSC-derived neurons exhibit a high degree of maturation and survive in culture for up to 4-5 months, even without astrocyte feeder layers.

Original languageEnglish (US)
Pages (from-to)993-1008
Number of pages16
JournalStem Cell Reports
Volume6
Issue number6
DOIs
StatePublished - Jun 14 2016
Externally publishedYes

Fingerprint

Pluripotent Stem Cells
Stem cells
Cell culture
Neurons
Suspensions
Cytology
Small Molecule Libraries
Feeder Cells
Developmental Biology
Depolarization
Neurology
Pathology
Human Body
Astrocytes
Synapses
Nervous System
Cell Biology
Screening
Experiments

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Rigamonti, A., Repetti, G. G., Sun, C., Price, F. D., Reny, D. C., Rapino, F., ... Rubin, L. L. (2016). Large-scale production of mature neurons from human pluripotent stem cells in a three-dimensional suspension culture system. Stem Cell Reports, 6(6), 993-1008. https://doi.org/10.1016/j.stemcr.2016.05.010

Large-scale production of mature neurons from human pluripotent stem cells in a three-dimensional suspension culture system. / Rigamonti, Alessandra; Repetti, Giuliana G.; Sun, Chicheng; Price, Feodor D.; Reny, Danielle C.; Rapino, Francesca; Weisinger, Karen; Benkler, Chen; Peterson, Quinn; Davidow, Lance S.; Hansson, Emil M.; Rubin, Lee L.

In: Stem Cell Reports, Vol. 6, No. 6, 14.06.2016, p. 993-1008.

Research output: Contribution to journalArticle

Rigamonti, A, Repetti, GG, Sun, C, Price, FD, Reny, DC, Rapino, F, Weisinger, K, Benkler, C, Peterson, Q, Davidow, LS, Hansson, EM & Rubin, LL 2016, 'Large-scale production of mature neurons from human pluripotent stem cells in a three-dimensional suspension culture system', Stem Cell Reports, vol. 6, no. 6, pp. 993-1008. https://doi.org/10.1016/j.stemcr.2016.05.010
Rigamonti, Alessandra ; Repetti, Giuliana G. ; Sun, Chicheng ; Price, Feodor D. ; Reny, Danielle C. ; Rapino, Francesca ; Weisinger, Karen ; Benkler, Chen ; Peterson, Quinn ; Davidow, Lance S. ; Hansson, Emil M. ; Rubin, Lee L. / Large-scale production of mature neurons from human pluripotent stem cells in a three-dimensional suspension culture system. In: Stem Cell Reports. 2016 ; Vol. 6, No. 6. pp. 993-1008.
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