@article{fb3ffbabf92244c3bbdf81e3913ed6f8,
title = "Large-scale production of mature neurons from human pluripotent stem cells in a three-dimensional suspension culture system",
abstract = "Human pluripotent stem cells (hPSCs) offer a renewable source of cells that can be expanded indefinitely and differentiated into virtually any type of cell in the human body, including neurons. This opens up unprecedented possibilities to study neuronal cell and developmental biology and cellular pathology of the nervous system, provides a platform for the screening of chemical libraries that affect these processes, and offers a potential source of transplantable cells for regenerative approaches to neurological disease. However, defining protocols that permit a large number and high yield of neurons has proved difficult. We present differentiation protocols for the generation of distinct subtypes of neurons in a highly reproducible manner, with minimal experiment-to-experiment variation. These neurons form synapses with neighboring cells, exhibit spontaneous electrical activity, and respond appropriately to depolarization. hPSC-derived neurons exhibit a high degree of maturation and survive in culture for up to 4-5 months, even without astrocyte feeder layers.",
author = "Alessandra Rigamonti and Repetti, {Giuliana G.} and Chicheng Sun and Price, {Feodor D.} and Reny, {Danielle C.} and Francesca Rapino and Karen Weisinger and Chen Benkler and Peterson, {Quinn P.} and Davidow, {Lance S.} and Hansson, {Emil M.} and Rubin, {Lee L.}",
note = "Funding Information: We would like to thank Douglas Melton for advice on the spinner method and for other helpful suggestions; Douglas Richardson from the Harvard Center for Biological Imaging (CBI) for assistance with LSFM; Gabriella Boulting and Bulent Ataman for helpful discussions; Laurence Daheron of the Harvard Stem Cell Institute iPS cell core and members of the Harvard Stem Cell Institute Histology Core for invaluable assistance; Jane Lalonde for editorial assistance; and Geraldine Jowett for technical help. We would also like to thank all the members of the Rubin laboratory for helpful discussions. We are grateful to Douglas Melton for providing the human ESCs HUES9, HUES8, and the 1016A human iPSCs; Kevin Eggan for the HUES3 hESCs; and Kenneth Chien for the H9ISL1RFP hESCs. BJ SiPSCs were generated by the HSCI iPSC Core. This work was supported by grants from the SMA Foundation , NINDS ( P01NS066888 ), Ellison Medical Foundation , Hoffman La-Roche, Inc. , the Stanley Center for Psychiatric Research at the Broad Institute, and the Harvard Stem Cell Institute . F.D.P. is a Banting Fellow and E.M.H. is a fellow of the Wenner-Gren Foundation. Publisher Copyright: {\textcopyright} 2016 The Author(s).",
year = "2016",
month = jun,
day = "14",
doi = "10.1016/j.stemcr.2016.05.010",
language = "English (US)",
volume = "6",
pages = "993--1008",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "6",
}