Large scale isolation of human erythrocyte membranes by high volume molecular filtration

Terrone L. Rosenberry, Jeffrey F. Chen, Mary M L Lee, Thomas A. Moulton, Philip Onigman

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A molecular filtration procedure for preparing large quantities of human erythrocyte ghost membranes is presented. Hemolysate ghost membranes are rapidly cycled in the retantate channel of the filtration apparatus, while hemoglobin is removed s it pass through Pellicon filters into the filtrate. Several-liter quantities of washed packed erythrocytes can be processed in a few hours with this system and the filtration procedure does not appear to alter erythrocyte or ghost membranes. Intact erythrocytes in isotonic solution can be circulated through the retentate channel for 16 h with only 3% hemolysis and with preservation of their orginal morphology in scanning electron microscopy. Ghost membranes isolated by the procedure are virtually identical in morphology, polypeptide composition and acetylcholinesterase content to membranes isolated by conventional centrifugation techniques.

Original languageEnglish (US)
Pages (from-to)39-48
Number of pages10
JournalJournal of Biochemical and Biophysical Methods
Volume4
Issue number1
DOIs
StatePublished - 1981
Externally publishedYes

Fingerprint

Erythrocyte Membrane
Membranes
Erythrocytes
Isotonic Solutions
Acetylcholinesterase
Hemolysis
Centrifugation
Electron Scanning Microscopy
Hemoglobins
Peptides
Scanning electron microscopy
Chemical analysis

Keywords

  • acetylcolinesterase
  • erythrocyte membranes
  • hemolysis
  • membrane filtration

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Large scale isolation of human erythrocyte membranes by high volume molecular filtration. / Rosenberry, Terrone L.; Chen, Jeffrey F.; Lee, Mary M L; Moulton, Thomas A.; Onigman, Philip.

In: Journal of Biochemical and Biophysical Methods, Vol. 4, No. 1, 1981, p. 39-48.

Research output: Contribution to journalArticle

Rosenberry, Terrone L. ; Chen, Jeffrey F. ; Lee, Mary M L ; Moulton, Thomas A. ; Onigman, Philip. / Large scale isolation of human erythrocyte membranes by high volume molecular filtration. In: Journal of Biochemical and Biophysical Methods. 1981 ; Vol. 4, No. 1. pp. 39-48.
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