Large-scale and comprehensive immune profiling and functional analysis of normal human aging

Chan C. Whiting, Janet Siebert, Aaron M. Newman, Hong Wu Du, Ash A. Alizadeh, Jorg Goronzy, Cornelia M. Weyand, Eswar Krishnan, C. Garrison Fathman, Holden T. Maecker

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

While many age-associated immune changes have been reported, a comprehensive set of metrics of immune aging is lacking. Here we report data from 243 healthy adults aged 40- 97, for whom we measured clinical and functional parameters, serum cytokines, cytokines and gene expression in stimulated and unstimulated PBMC, PBMC phenotypes, and cytokine- stimulated pSTAT signaling in whole blood. Although highly heterogeneous across individuals, many of these assays revealed trends by age, sex, and CMV status, to greater or lesser degrees. Age, then sex and CMV status, showed the greatest impact on the immune system, as measured by the percentage of assay readouts with significant differences. An elastic net regression model could optimally predict age with 14 analytes from different assays. This reinforces the importance of multivariate analysis for defining a healthy immune system. These data provide a reference for others measuring immune parameters in older people.

Original languageEnglish (US)
Article numbere0133627
JournalPloS one
Volume10
Issue number7
DOIs
StatePublished - Jul 21 2015

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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