Laminotomy for lumbar dorsal root ganglion access and injection in swine

Mark D. Unger, Timothy Maus, Ross C. Puffer, Laura K. Newman, Bradford L. Currier, Andreas S Beutler

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Dorsal root ganglia (DRG) are anatomically well defined structures that contain all primary sensory neurons below the head. This fact makes DRG attractive targets for injection of novel therapeutics aimed at treating chronic pain. In small animal models, laminectomy has been used to facilitate DRG injection because it involves surgical removal of the vertebral bone surrounding each DRG. We demonstrate a technique for intraganglionic injection of lumbar DRG in a large animal species, namely, swine. Laminotomy is performed to allow direct access to DRG using standard neurosurgical techniques, instruments, and materials. Compared with more extensive bone removal via laminectomy, we implement laminotomy to conserve spinal anatomy while achieving sufficient DRG access. Intraoperative progress of DRG injection is monitored using a non-toxic dye. Following euthanasia on post-operative day 21, the success of injection is determined by histology for intraganglionic distribution of 4',6-diamidino-2-phenylindole (DAPI). We inject a biologically inactive solution to demonstrate the protocol. This method could be applied in future preclinical studies to target therapeutic solutions to DRG. Our methodology should facilitate testing the translatability of intraganglionic small animal paradigms in a large animal species. Additionally, this protocol may serve as a key resource for those planning preclinical studies of DRG injection in swine.

Original languageEnglish (US)
Article numbere56434
JournalJournal of Visualized Experiments
Volume2017
Issue number128
DOIs
StatePublished - Oct 10 2017

Fingerprint

Laminectomy
Spinal Ganglia
Animals
Swine
Injections
Bone
Histology
Neurons
Coloring Agents
Dyes
Planning
Testing
Bone and Bones
Euthanasia
Sensory Receptor Cells
Chronic Pain
Anatomy
Animal Models
Head

Keywords

  • Convection enhanced delivery
  • Dorsal root ganglion
  • Intraganglionic injection
  • Laminotomy
  • Large animal
  • Swine

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Laminotomy for lumbar dorsal root ganglion access and injection in swine. / Unger, Mark D.; Maus, Timothy; Puffer, Ross C.; Newman, Laura K.; Currier, Bradford L.; Beutler, Andreas S.

In: Journal of Visualized Experiments, Vol. 2017, No. 128, e56434, 10.10.2017.

Research output: Contribution to journalArticle

Unger, Mark D. ; Maus, Timothy ; Puffer, Ross C. ; Newman, Laura K. ; Currier, Bradford L. ; Beutler, Andreas S. / Laminotomy for lumbar dorsal root ganglion access and injection in swine. In: Journal of Visualized Experiments. 2017 ; Vol. 2017, No. 128.
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