Lactoferricin mediates anti-inflammatory and anti-catabolic effects via inhibition of IL-1 and LPS activity in the intervertebral disc

Jae Sung Kim, Michael B. Ellman, Dongyao Yan, Howard S. An, Ranjan Kc, Xin Li, Di Chen, Guozhi Xiao, Gabriella Cs-Szabo, David W. Hoskin, Doug D. Buechter, Andre J. Van Wijnen, Hee Jeong Im

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

The catabolic cytokine interleukin-1 (IL-1) and endotoxin lipopolysaccharide (LPS) are well-known inflammatory mediators involved in degenerative disc disease, and inhibitors of IL-1 and LPS may potentially be used to slow or prevent disc degeneration in vivo. Here, we elucidate the striking anti-catabolic and anti-inflammatory effects of bovine lactoferricin (LfcinB) in the intervertebral disc (IVD) via antagonism of both IL-1 and LPS-mediated catabolic activity using in vitro and ex vivo analyses. Specifically, we demonstrate the biological counteraction of LfcinB against IL-1 and LPS-mediated proteoglycan (PG) depletion, matrix-degrading enzyme production, and enzyme activity in long-term (alginate beads) and short-term (monolayer) culture models using bovine and human nucleus pulposus (NP) cells. LfcinB significantly attenuates the IL-1 and LPS-mediated suppression of PG production and synthesis, and thus restores PG accumulation and pericellular matrix formation. Simultaneously, LfcinB antagonizes catabolic factor mediated induction of multiple cartilage-degrading enzymes, including MMP-1, MMP-3, MMP-13, ADAMTS-4, and ADAMTS-5, in bovine NP cells at both mRNA and protein levels. LfcinB also suppresses the catabolic factor-induced stimulation of oxidative and inflammatory factors such as iNOS, IL-6, and toll-like receptor-2 (TLR-2) and TLR-4. Finally, the ability of LfcinB to antagonize IL-1 and LPS-mediated suppression of PG is upheld in an en bloc intradiscal microinjection model followed by ex vivo organ culture using both mouse and rabbit IVD tissue, suggesting a potential therapeutic benefit of LfcinB on degenerative disc disease in the future.

Original languageEnglish (US)
Pages (from-to)1884-1896
Number of pages13
JournalJournal of Cellular Physiology
Volume228
Issue number9
DOIs
StatePublished - Sep 2013

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Lactoferricin mediates anti-inflammatory and anti-catabolic effects via inhibition of IL-1 and LPS activity in the intervertebral disc'. Together they form a unique fingerprint.

  • Cite this

    Kim, J. S., Ellman, M. B., Yan, D., An, H. S., Kc, R., Li, X., Chen, D., Xiao, G., Cs-Szabo, G., Hoskin, D. W., Buechter, D. D., Van Wijnen, A. J., & Im, H. J. (2013). Lactoferricin mediates anti-inflammatory and anti-catabolic effects via inhibition of IL-1 and LPS activity in the intervertebral disc. Journal of Cellular Physiology, 228(9), 1884-1896. https://doi.org/10.1002/jcp.24350