Lack of functionally active sweet taste receptors in the jejunum in vivo in the rat

Rizwan M. Chaudhry, Alok Garg, Mohamed M. Abdelfatah, Judith A. Duenes, Michael G. Sarr

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: When studied in enterocyte-like cell lines (Caco-2 and RIE cells), agonists and antagonists of the sweet taste receptor (STR) augment and decrease glucose uptake, respectively. We hypothesize that exposure to STR agonists and antagonists in vivo will augment glucose absorption in the rat. Materials and methods: About 30-cm segments of jejunum in anesthetized rats were perfused with iso-osmolar solutions containing 10, 35, and 100 mM glucose solutions (n = 6 rats, each group) with and without the STR agonist 2 mM acesulfame potassium and the STR inhibitor 10 μM U-73122 (inhibitor of the phospholipase C pathway). Carrier-mediated absorption of glucose was calculated by using stereospecific and nonstereospecific 14C-d-glucose and 3H-l-glucose, respectively. Results: Addition of the STR agonist acesulfame potassium to the 10, 35, and 100 mM glucose solutions had no substantive effects on glucose absorption from 2.1 ± 0.2 to 2.0 ± 0.3, 5.8 ± 0.2 to 4.8 ± 0.2, and 15.5 ± 2.3 to 15.7 ± 2.7 μmoL/min/30-cm intestinal segment (P > 0.05), respectively. Addition of the STR inhibitor (U-73122) also had no effect on absorption in the 10, 35, and 100 mM solutions from 2.3 ± 0.1 to 2.1 ± 0.2, 7.7 ± 0.5 to 7.2 ± 0.5, and 15.7 ± 0.9 to 15.2 ± 1.1 μmoL/min/30-cm intestinal segment, respectively. Conclusions: Provision of glucose directly into rat jejunum does not augment glucose absorption via STR-mediated mechanisms within the jejunum in the rat. Our experiments show either no major role of STRs in mediating postprandial augmentation of glucose absorption or that proximal gastrointestinal tract stimulation of STR or other luminal factors may be required for absorption of glucose to be augmented by STR.

Original languageEnglish (US)
Pages (from-to)606-611
Number of pages6
JournalJournal of Surgical Research
Volume183
Issue number2
DOIs
StatePublished - Aug 2013

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Jejunum
Glucose
Caco-2 Cells
Enterocytes
Type C Phospholipases
Gastrointestinal Tract
Cell Line

Keywords

  • Acesulfame potassium
  • Apical translocation
  • Glucose absorption
  • GLUT2
  • SGLT1
  • Sweet taste receptors
  • U73122

ASJC Scopus subject areas

  • Surgery

Cite this

Chaudhry, R. M., Garg, A., Abdelfatah, M. M., Duenes, J. A., & Sarr, M. G. (2013). Lack of functionally active sweet taste receptors in the jejunum in vivo in the rat. Journal of Surgical Research, 183(2), 606-611. https://doi.org/10.1016/j.jss.2013.02.031

Lack of functionally active sweet taste receptors in the jejunum in vivo in the rat. / Chaudhry, Rizwan M.; Garg, Alok; Abdelfatah, Mohamed M.; Duenes, Judith A.; Sarr, Michael G.

In: Journal of Surgical Research, Vol. 183, No. 2, 08.2013, p. 606-611.

Research output: Contribution to journalArticle

Chaudhry, RM, Garg, A, Abdelfatah, MM, Duenes, JA & Sarr, MG 2013, 'Lack of functionally active sweet taste receptors in the jejunum in vivo in the rat', Journal of Surgical Research, vol. 183, no. 2, pp. 606-611. https://doi.org/10.1016/j.jss.2013.02.031
Chaudhry, Rizwan M. ; Garg, Alok ; Abdelfatah, Mohamed M. ; Duenes, Judith A. ; Sarr, Michael G. / Lack of functionally active sweet taste receptors in the jejunum in vivo in the rat. In: Journal of Surgical Research. 2013 ; Vol. 183, No. 2. pp. 606-611.
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abstract = "Background: When studied in enterocyte-like cell lines (Caco-2 and RIE cells), agonists and antagonists of the sweet taste receptor (STR) augment and decrease glucose uptake, respectively. We hypothesize that exposure to STR agonists and antagonists in vivo will augment glucose absorption in the rat. Materials and methods: About 30-cm segments of jejunum in anesthetized rats were perfused with iso-osmolar solutions containing 10, 35, and 100 mM glucose solutions (n = 6 rats, each group) with and without the STR agonist 2 mM acesulfame potassium and the STR inhibitor 10 μM U-73122 (inhibitor of the phospholipase C pathway). Carrier-mediated absorption of glucose was calculated by using stereospecific and nonstereospecific 14C-d-glucose and 3H-l-glucose, respectively. Results: Addition of the STR agonist acesulfame potassium to the 10, 35, and 100 mM glucose solutions had no substantive effects on glucose absorption from 2.1 ± 0.2 to 2.0 ± 0.3, 5.8 ± 0.2 to 4.8 ± 0.2, and 15.5 ± 2.3 to 15.7 ± 2.7 μmoL/min/30-cm intestinal segment (P > 0.05), respectively. Addition of the STR inhibitor (U-73122) also had no effect on absorption in the 10, 35, and 100 mM solutions from 2.3 ± 0.1 to 2.1 ± 0.2, 7.7 ± 0.5 to 7.2 ± 0.5, and 15.7 ± 0.9 to 15.2 ± 1.1 μmoL/min/30-cm intestinal segment, respectively. Conclusions: Provision of glucose directly into rat jejunum does not augment glucose absorption via STR-mediated mechanisms within the jejunum in the rat. Our experiments show either no major role of STRs in mediating postprandial augmentation of glucose absorption or that proximal gastrointestinal tract stimulation of STR or other luminal factors may be required for absorption of glucose to be augmented by STR.",
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AU - Duenes, Judith A.

AU - Sarr, Michael G.

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N2 - Background: When studied in enterocyte-like cell lines (Caco-2 and RIE cells), agonists and antagonists of the sweet taste receptor (STR) augment and decrease glucose uptake, respectively. We hypothesize that exposure to STR agonists and antagonists in vivo will augment glucose absorption in the rat. Materials and methods: About 30-cm segments of jejunum in anesthetized rats were perfused with iso-osmolar solutions containing 10, 35, and 100 mM glucose solutions (n = 6 rats, each group) with and without the STR agonist 2 mM acesulfame potassium and the STR inhibitor 10 μM U-73122 (inhibitor of the phospholipase C pathway). Carrier-mediated absorption of glucose was calculated by using stereospecific and nonstereospecific 14C-d-glucose and 3H-l-glucose, respectively. Results: Addition of the STR agonist acesulfame potassium to the 10, 35, and 100 mM glucose solutions had no substantive effects on glucose absorption from 2.1 ± 0.2 to 2.0 ± 0.3, 5.8 ± 0.2 to 4.8 ± 0.2, and 15.5 ± 2.3 to 15.7 ± 2.7 μmoL/min/30-cm intestinal segment (P > 0.05), respectively. Addition of the STR inhibitor (U-73122) also had no effect on absorption in the 10, 35, and 100 mM solutions from 2.3 ± 0.1 to 2.1 ± 0.2, 7.7 ± 0.5 to 7.2 ± 0.5, and 15.7 ± 0.9 to 15.2 ± 1.1 μmoL/min/30-cm intestinal segment, respectively. Conclusions: Provision of glucose directly into rat jejunum does not augment glucose absorption via STR-mediated mechanisms within the jejunum in the rat. Our experiments show either no major role of STRs in mediating postprandial augmentation of glucose absorption or that proximal gastrointestinal tract stimulation of STR or other luminal factors may be required for absorption of glucose to be augmented by STR.

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