Lack of detection of enteroviral RNA or bacterial DNA in magnetic resonance imaging-directed muscle biopsies from twenty children with active untreated juvenile dermatomyositis

Lauren M. Pachman, Deborah L. Litt, Anne H. Rowley, Jennifer R. Hayford, Janice Caliendo, Scott Heller, Baruch S. Ticho, Mary Christensen, Bruce Patterson, Steven R Ytterberg, Mark Pallansch

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Objective. To investigate for the presence of increased titers of circulating antibody to putative infectious agents and for detectable viral RNA or bacterial DNA in children with active recent-onset juvenile dermatomyositis (DM). Methods. Magnetic resonance imaging-directed muscle biopsies were performed in 20 children with active, untreated, recent-onset juvenile DM and in age-matched children with neurologic disease. Sera were tested for complement-fixing antibody to Coxsackievirus B (CVB), influenza A and B, parainfluenza 1 and 3, Mycoplasma pneumoniae, mumps, respiratory syncytial virus, and Reovirus; and by immunofluorescence for IgG antibody to Toxoplasma gondii cytomegalovirus and IgM antibody to Epstein-Barr virus. Muscle from juvenile DM patients and control children, CD-1 Swiss mice with and without CVB1 infection, and viral stock positive for CVB1-6 were tested using reverse-transcriptase polymerase chain reaction with 5 primer sets, 4 probes (1 Coxsackievirus, 3 Enterovirus), and universal primers for DNA. Results. No increased antibody, viral RNA, or bacterial DNA was present in the juvenile DM patients or the control children. Conclusion. Juvenile DM may be triggered by unidentified agent(s) in the genetically susceptible host.

Original languageEnglish (US)
Pages (from-to)1513-1518
Number of pages6
JournalArthritis and Rheumatism
Volume38
Issue number10
DOIs
StatePublished - Oct 1995
Externally publishedYes

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Bacterial DNA
Magnetic Resonance Imaging
RNA
Biopsy
Muscles
Antibodies
Enterovirus
Viral DNA
Viral RNA
Human Enterovirus B
Paramyxoviridae Infections
Mycoplasma pneumoniae
Mumps
DNA Primers
Respiratory Syncytial Viruses
Toxoplasma
Virus Diseases
Nervous System Diseases
Reverse Transcriptase Polymerase Chain Reaction
Cytomegalovirus

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Lack of detection of enteroviral RNA or bacterial DNA in magnetic resonance imaging-directed muscle biopsies from twenty children with active untreated juvenile dermatomyositis. / Pachman, Lauren M.; Litt, Deborah L.; Rowley, Anne H.; Hayford, Jennifer R.; Caliendo, Janice; Heller, Scott; Ticho, Baruch S.; Christensen, Mary; Patterson, Bruce; Ytterberg, Steven R; Pallansch, Mark.

In: Arthritis and Rheumatism, Vol. 38, No. 10, 10.1995, p. 1513-1518.

Research output: Contribution to journalArticle

Pachman, LM, Litt, DL, Rowley, AH, Hayford, JR, Caliendo, J, Heller, S, Ticho, BS, Christensen, M, Patterson, B, Ytterberg, SR & Pallansch, M 1995, 'Lack of detection of enteroviral RNA or bacterial DNA in magnetic resonance imaging-directed muscle biopsies from twenty children with active untreated juvenile dermatomyositis', Arthritis and Rheumatism, vol. 38, no. 10, pp. 1513-1518. https://doi.org/10.1002/art.1780381019
Pachman, Lauren M. ; Litt, Deborah L. ; Rowley, Anne H. ; Hayford, Jennifer R. ; Caliendo, Janice ; Heller, Scott ; Ticho, Baruch S. ; Christensen, Mary ; Patterson, Bruce ; Ytterberg, Steven R ; Pallansch, Mark. / Lack of detection of enteroviral RNA or bacterial DNA in magnetic resonance imaging-directed muscle biopsies from twenty children with active untreated juvenile dermatomyositis. In: Arthritis and Rheumatism. 1995 ; Vol. 38, No. 10. pp. 1513-1518.
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abstract = "Objective. To investigate for the presence of increased titers of circulating antibody to putative infectious agents and for detectable viral RNA or bacterial DNA in children with active recent-onset juvenile dermatomyositis (DM). Methods. Magnetic resonance imaging-directed muscle biopsies were performed in 20 children with active, untreated, recent-onset juvenile DM and in age-matched children with neurologic disease. Sera were tested for complement-fixing antibody to Coxsackievirus B (CVB), influenza A and B, parainfluenza 1 and 3, Mycoplasma pneumoniae, mumps, respiratory syncytial virus, and Reovirus; and by immunofluorescence for IgG antibody to Toxoplasma gondii cytomegalovirus and IgM antibody to Epstein-Barr virus. Muscle from juvenile DM patients and control children, CD-1 Swiss mice with and without CVB1 infection, and viral stock positive for CVB1-6 were tested using reverse-transcriptase polymerase chain reaction with 5 primer sets, 4 probes (1 Coxsackievirus, 3 Enterovirus), and universal primers for DNA. Results. No increased antibody, viral RNA, or bacterial DNA was present in the juvenile DM patients or the control children. Conclusion. Juvenile DM may be triggered by unidentified agent(s) in the genetically susceptible host.",
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AU - Caliendo, Janice

AU - Heller, Scott

AU - Ticho, Baruch S.

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AU - Pallansch, Mark

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