Lack of Association of the Ala 45Thr Polymorphism and Other Common Variants of the NeuroD Gene with Type 1 Diabetes

Adrian Vella, Joanna M.M. Howson, Bryan J. Barratt, Rebecca C.J. Twells, Helen E. Rance, Sarah Nutland, Eva Tuomilehto-Wolf, Jaakko Tuomilehto, Dag E. Undlien, Kjersti S. Rønningen, Cristian Guja, Constantin Ionescu-Tîrgovişte, David A. Savage, John A. Todd

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Variation in genes necessary for normal functioning and development of β-cells, e.g., NEUROD1, which encodes a transcription factor for the insulin gene and is important in β-cell development, causes maturity-onset diabetes of the young. Some studies have reported an association between a nonsynonymous Ala45Thr (+182G→ A) single nucleotide polymorphism (SNP) in NEUROD1 and type 1 diabetes, but this result has not been consistently found. To clarify this, we genotyped Ala45Thr in 2,434 type 1 diabetic families of European descent and Caucasian ethnicity from five different countries. Taking the allele frequency of 36% for Thr45 and an odds ratio (OR) of 1.2, this sample provided >99% power to detect an association (P < 0.95). We could not confirm the association (P = 0.77). No evidence of population heterogeneity in the lack of association of Thr 45 with type 1 diabetes was observed. To evaluate the possibility that another NEUROD1 variant was associated with type 1 diabetes, we resequenced the gene in 32 U.K. affected individuals and identified and genotyped all common SNPs (minor allele frequency >10%; n = 5) in 786 families. We report no evidence of association of these common variants in NEUROD1 and type 1 diabetes in these samples.

Original languageEnglish (US)
Pages (from-to)1158-1161
Number of pages4
JournalDiabetes
Volume53
Issue number4
DOIs
StatePublished - Apr 2004

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

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    Vella, A., Howson, J. M. M., Barratt, B. J., Twells, R. C. J., Rance, H. E., Nutland, S., Tuomilehto-Wolf, E., Tuomilehto, J., Undlien, D. E., Rønningen, K. S., Guja, C., Ionescu-Tîrgovişte, C., Savage, D. A., & Todd, J. A. (2004). Lack of Association of the Ala 45Thr Polymorphism and Other Common Variants of the NeuroD Gene with Type 1 Diabetes. Diabetes, 53(4), 1158-1161. https://doi.org/10.2337/diabetes.53.4.1158