Lack of association between transporter associated with antigen processing (TAP) and HLA-DM gene polymorphisms and antibody levels following measles vaccination

N. Dhiman, I. G. Ovsyannikova, N. A. Pinsky, R. A. Vierkant, S. J. Jacobsen, R. M. Jacobson, Gregory A. Poland

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The transporter associated with antigen processing (TAP) and human leukocyte antigen-DM (HLA-DM) genes are involved in the antigen-processing pathway of both HLA class I and class II-restricted antigen presentation. We hypothesized that polymorphisms within the TAP and DM genes may influence antibody levels following measles vaccination. We examined TAP and DM polymorphisms in 242 school children from Olmsted County, Minnesota, USA who received one dose of measles-mumps-rubella-II (MMR-II) vaccine at the age of 15 months. Based on the level of serum measles-specific immunoglobulin G (IgG) antibodies, subjects were classified as seronegatives (n = 72) or seropositives (n = 170). We determined TAP1 and TAP2 allele types by polymerase chain reaction (PCR) amplification of specific alleles (PASA) and determined DM allele type by PCR amplification followed by direct sequencing of the polymorphic sites. We analysed the data for any TAP or DM allelic association with antibody levels post measles vaccination using the chi-square test and univariate linear regression analysis. We found no trend in the overall distribution of TAP and DM genotype frequencies between seronegative and seropositive subjects, suggesting that TAP and DM polymorphism and antibody levels following measles vaccination are not directly associated. In addition, we did not find an association between TAP (TAP1, P = 0.71; TAP2, P = 0.87) or DM (DMA, P = 0.42; DMB, P = 0.71) homozygosity and seronegativity to measles vaccine in this study group. Our study suggests that TAP and DM gene polymorphisms do not influence antibody levels post measles vaccination.

Original languageEnglish (US)
Pages (from-to)195-200
Number of pages6
JournalEuropean Journal of Immunogenetics
Volume30
Issue number3
DOIs
StatePublished - Jun 2003

Fingerprint

Measles
HLA Antigens
Vaccination
Antibodies
Genes
Alleles
Antigen Presentation
Measles-Mumps-Rubella Vaccine
Measles Vaccine
Polymerase Chain Reaction
Histocompatibility Antigens Class II
Chi-Square Distribution
Linear Models
Immunoglobulin G
Genotype
Regression Analysis
transporter associated with antigen processing (TAP)
Serum

ASJC Scopus subject areas

  • Genetics
  • Immunology

Cite this

Lack of association between transporter associated with antigen processing (TAP) and HLA-DM gene polymorphisms and antibody levels following measles vaccination. / Dhiman, N.; Ovsyannikova, I. G.; Pinsky, N. A.; Vierkant, R. A.; Jacobsen, S. J.; Jacobson, R. M.; Poland, Gregory A.

In: European Journal of Immunogenetics, Vol. 30, No. 3, 06.2003, p. 195-200.

Research output: Contribution to journalArticle

Dhiman, N. ; Ovsyannikova, I. G. ; Pinsky, N. A. ; Vierkant, R. A. ; Jacobsen, S. J. ; Jacobson, R. M. ; Poland, Gregory A. / Lack of association between transporter associated with antigen processing (TAP) and HLA-DM gene polymorphisms and antibody levels following measles vaccination. In: European Journal of Immunogenetics. 2003 ; Vol. 30, No. 3. pp. 195-200.
@article{971fd5e2320741aebb0d456e128ab33d,
title = "Lack of association between transporter associated with antigen processing (TAP) and HLA-DM gene polymorphisms and antibody levels following measles vaccination",
abstract = "The transporter associated with antigen processing (TAP) and human leukocyte antigen-DM (HLA-DM) genes are involved in the antigen-processing pathway of both HLA class I and class II-restricted antigen presentation. We hypothesized that polymorphisms within the TAP and DM genes may influence antibody levels following measles vaccination. We examined TAP and DM polymorphisms in 242 school children from Olmsted County, Minnesota, USA who received one dose of measles-mumps-rubella-II (MMR-II) vaccine at the age of 15 months. Based on the level of serum measles-specific immunoglobulin G (IgG) antibodies, subjects were classified as seronegatives (n = 72) or seropositives (n = 170). We determined TAP1 and TAP2 allele types by polymerase chain reaction (PCR) amplification of specific alleles (PASA) and determined DM allele type by PCR amplification followed by direct sequencing of the polymorphic sites. We analysed the data for any TAP or DM allelic association with antibody levels post measles vaccination using the chi-square test and univariate linear regression analysis. We found no trend in the overall distribution of TAP and DM genotype frequencies between seronegative and seropositive subjects, suggesting that TAP and DM polymorphism and antibody levels following measles vaccination are not directly associated. In addition, we did not find an association between TAP (TAP1, P = 0.71; TAP2, P = 0.87) or DM (DMA, P = 0.42; DMB, P = 0.71) homozygosity and seronegativity to measles vaccine in this study group. Our study suggests that TAP and DM gene polymorphisms do not influence antibody levels post measles vaccination.",
author = "N. Dhiman and Ovsyannikova, {I. G.} and Pinsky, {N. A.} and Vierkant, {R. A.} and Jacobsen, {S. J.} and Jacobson, {R. M.} and Poland, {Gregory A.}",
year = "2003",
month = "6",
doi = "10.1046/j.1365-2370.2003.00382.x",
language = "English (US)",
volume = "30",
pages = "195--200",
journal = "International Journal of Immunogenetics",
issn = "1744-3121",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Lack of association between transporter associated with antigen processing (TAP) and HLA-DM gene polymorphisms and antibody levels following measles vaccination

AU - Dhiman, N.

AU - Ovsyannikova, I. G.

AU - Pinsky, N. A.

AU - Vierkant, R. A.

AU - Jacobsen, S. J.

AU - Jacobson, R. M.

AU - Poland, Gregory A.

PY - 2003/6

Y1 - 2003/6

N2 - The transporter associated with antigen processing (TAP) and human leukocyte antigen-DM (HLA-DM) genes are involved in the antigen-processing pathway of both HLA class I and class II-restricted antigen presentation. We hypothesized that polymorphisms within the TAP and DM genes may influence antibody levels following measles vaccination. We examined TAP and DM polymorphisms in 242 school children from Olmsted County, Minnesota, USA who received one dose of measles-mumps-rubella-II (MMR-II) vaccine at the age of 15 months. Based on the level of serum measles-specific immunoglobulin G (IgG) antibodies, subjects were classified as seronegatives (n = 72) or seropositives (n = 170). We determined TAP1 and TAP2 allele types by polymerase chain reaction (PCR) amplification of specific alleles (PASA) and determined DM allele type by PCR amplification followed by direct sequencing of the polymorphic sites. We analysed the data for any TAP or DM allelic association with antibody levels post measles vaccination using the chi-square test and univariate linear regression analysis. We found no trend in the overall distribution of TAP and DM genotype frequencies between seronegative and seropositive subjects, suggesting that TAP and DM polymorphism and antibody levels following measles vaccination are not directly associated. In addition, we did not find an association between TAP (TAP1, P = 0.71; TAP2, P = 0.87) or DM (DMA, P = 0.42; DMB, P = 0.71) homozygosity and seronegativity to measles vaccine in this study group. Our study suggests that TAP and DM gene polymorphisms do not influence antibody levels post measles vaccination.

AB - The transporter associated with antigen processing (TAP) and human leukocyte antigen-DM (HLA-DM) genes are involved in the antigen-processing pathway of both HLA class I and class II-restricted antigen presentation. We hypothesized that polymorphisms within the TAP and DM genes may influence antibody levels following measles vaccination. We examined TAP and DM polymorphisms in 242 school children from Olmsted County, Minnesota, USA who received one dose of measles-mumps-rubella-II (MMR-II) vaccine at the age of 15 months. Based on the level of serum measles-specific immunoglobulin G (IgG) antibodies, subjects were classified as seronegatives (n = 72) or seropositives (n = 170). We determined TAP1 and TAP2 allele types by polymerase chain reaction (PCR) amplification of specific alleles (PASA) and determined DM allele type by PCR amplification followed by direct sequencing of the polymorphic sites. We analysed the data for any TAP or DM allelic association with antibody levels post measles vaccination using the chi-square test and univariate linear regression analysis. We found no trend in the overall distribution of TAP and DM genotype frequencies between seronegative and seropositive subjects, suggesting that TAP and DM polymorphism and antibody levels following measles vaccination are not directly associated. In addition, we did not find an association between TAP (TAP1, P = 0.71; TAP2, P = 0.87) or DM (DMA, P = 0.42; DMB, P = 0.71) homozygosity and seronegativity to measles vaccine in this study group. Our study suggests that TAP and DM gene polymorphisms do not influence antibody levels post measles vaccination.

UR - http://www.scopus.com/inward/record.url?scp=0037636127&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037636127&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2370.2003.00382.x

DO - 10.1046/j.1365-2370.2003.00382.x

M3 - Article

VL - 30

SP - 195

EP - 200

JO - International Journal of Immunogenetics

JF - International Journal of Immunogenetics

SN - 1744-3121

IS - 3

ER -