Lack of acrosome formation in Hrb-deficient mice

N. Kang-Decker, G. T. Mantchev, S. C. Juneja, Mark A Mc Niven, Jan Van Deursen

Research output: Contribution to journalArticle

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Abstract

The sperm acrosome is essential for sperm-egg fusion and is often defective in men with nonobstructive infertility. Here we report that male mice with a null mutation in Hrb are infertile and display round-headed spermatozoa that lack an acrosome. In wild-type spermatids, Hrb, is associated with the cytosolic surface of proacrosomic transport vesicles that fuse to create a single large acrosomic vesicle at step 3 of spermiogenesis. Although proacrosomic vesicles form in spermatids that lack Hrb, the vesicles are unable to fuse, blocking acrosome development at step 2. We conclude that Hrb is required for docking and/or fusion of proacrosomic vesicles during acrosome biogenesis.

Original languageEnglish (US)
Pages (from-to)1531-1533
Number of pages3
JournalScience
Volume294
Issue number5546
DOIs
StatePublished - Nov 16 2001

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Acrosome
Spermatozoa
Spermatids
Transport Vesicles
Spermatogenesis
Infertility
Ovum
Mutation

ASJC Scopus subject areas

  • General

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Lack of acrosome formation in Hrb-deficient mice. / Kang-Decker, N.; Mantchev, G. T.; Juneja, S. C.; Mc Niven, Mark A; Van Deursen, Jan.

In: Science, Vol. 294, No. 5546, 16.11.2001, p. 1531-1533.

Research output: Contribution to journalArticle

Kang-Decker, N, Mantchev, GT, Juneja, SC, Mc Niven, MA & Van Deursen, J 2001, 'Lack of acrosome formation in Hrb-deficient mice', Science, vol. 294, no. 5546, pp. 1531-1533. https://doi.org/10.1126/science.1063665
Kang-Decker, N. ; Mantchev, G. T. ; Juneja, S. C. ; Mc Niven, Mark A ; Van Deursen, Jan. / Lack of acrosome formation in Hrb-deficient mice. In: Science. 2001 ; Vol. 294, No. 5546. pp. 1531-1533.
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