Laboratory diagnosis of infective endocarditis

Rachael M. Liesman, Bobbi S. Pritt, Joseph Maleszewski, Robin Patel

Research output: Contribution to journalReview article

20 Citations (Scopus)

Abstract

Infective endocarditis is life-threatening; identification of the underlying etiology informs optimized individual patient management. Changing epidemiology, advances in blood culture techniques, and new diagnostics guide the application of laboratory testing for diagnosis of endocarditis. Blood cultures remain the standard test for microbial diagnosis, with directed serological testing (i.e., Q fever serology, Bartonella serology) in culture-negative cases. Histopathology and molecular diagnostics (e.g., 16S rRNA gene PCR/sequencing, Tropheryma whipplei PCR) may be applied to resected valves to aid in diagnosis. Herein, we summarize recent knowledge in this area and propose a microbiologic and pathological algorithm for endocarditis diagnosis.

Original languageEnglish (US)
Pages (from-to)2599-2608
Number of pages10
JournalJournal of Clinical Microbiology
Volume55
Issue number9
DOIs
StatePublished - Sep 1 2017

Fingerprint

Clinical Laboratory Techniques
Endocarditis
Serology
Tropheryma
Bartonella
Q Fever
Culture Techniques
Polymerase Chain Reaction
Molecular Pathology
rRNA Genes
Epidemiology
Blood Culture

Keywords

  • Clinical microbiology
  • Endocarditis

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Laboratory diagnosis of infective endocarditis. / Liesman, Rachael M.; Pritt, Bobbi S.; Maleszewski, Joseph; Patel, Robin.

In: Journal of Clinical Microbiology, Vol. 55, No. 9, 01.09.2017, p. 2599-2608.

Research output: Contribution to journalReview article

Liesman, Rachael M. ; Pritt, Bobbi S. ; Maleszewski, Joseph ; Patel, Robin. / Laboratory diagnosis of infective endocarditis. In: Journal of Clinical Microbiology. 2017 ; Vol. 55, No. 9. pp. 2599-2608.
@article{29494fc43f1145dbb9911c4d10c784d5,
title = "Laboratory diagnosis of infective endocarditis",
abstract = "Infective endocarditis is life-threatening; identification of the underlying etiology informs optimized individual patient management. Changing epidemiology, advances in blood culture techniques, and new diagnostics guide the application of laboratory testing for diagnosis of endocarditis. Blood cultures remain the standard test for microbial diagnosis, with directed serological testing (i.e., Q fever serology, Bartonella serology) in culture-negative cases. Histopathology and molecular diagnostics (e.g., 16S rRNA gene PCR/sequencing, Tropheryma whipplei PCR) may be applied to resected valves to aid in diagnosis. Herein, we summarize recent knowledge in this area and propose a microbiologic and pathological algorithm for endocarditis diagnosis.",
keywords = "Clinical microbiology, Endocarditis",
author = "Liesman, {Rachael M.} and Pritt, {Bobbi S.} and Joseph Maleszewski and Robin Patel",
year = "2017",
month = "9",
day = "1",
doi = "10.1128/JCM.00635-17",
language = "English (US)",
volume = "55",
pages = "2599--2608",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "9",

}

TY - JOUR

T1 - Laboratory diagnosis of infective endocarditis

AU - Liesman, Rachael M.

AU - Pritt, Bobbi S.

AU - Maleszewski, Joseph

AU - Patel, Robin

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Infective endocarditis is life-threatening; identification of the underlying etiology informs optimized individual patient management. Changing epidemiology, advances in blood culture techniques, and new diagnostics guide the application of laboratory testing for diagnosis of endocarditis. Blood cultures remain the standard test for microbial diagnosis, with directed serological testing (i.e., Q fever serology, Bartonella serology) in culture-negative cases. Histopathology and molecular diagnostics (e.g., 16S rRNA gene PCR/sequencing, Tropheryma whipplei PCR) may be applied to resected valves to aid in diagnosis. Herein, we summarize recent knowledge in this area and propose a microbiologic and pathological algorithm for endocarditis diagnosis.

AB - Infective endocarditis is life-threatening; identification of the underlying etiology informs optimized individual patient management. Changing epidemiology, advances in blood culture techniques, and new diagnostics guide the application of laboratory testing for diagnosis of endocarditis. Blood cultures remain the standard test for microbial diagnosis, with directed serological testing (i.e., Q fever serology, Bartonella serology) in culture-negative cases. Histopathology and molecular diagnostics (e.g., 16S rRNA gene PCR/sequencing, Tropheryma whipplei PCR) may be applied to resected valves to aid in diagnosis. Herein, we summarize recent knowledge in this area and propose a microbiologic and pathological algorithm for endocarditis diagnosis.

KW - Clinical microbiology

KW - Endocarditis

UR - http://www.scopus.com/inward/record.url?scp=85028306342&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85028306342&partnerID=8YFLogxK

U2 - 10.1128/JCM.00635-17

DO - 10.1128/JCM.00635-17

M3 - Review article

C2 - 28659319

AN - SCOPUS:85028306342

VL - 55

SP - 2599

EP - 2608

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 9

ER -