@article{3980e2e99e114d07b2b458030da148c0,
title = "Label-Free Isothermal Amplification Assay for Specific and Highly Sensitive Colorimetric miRNA Detection",
abstract = "We describe a new method for the detection of miRNA in biological samples. This technology is based on the isothermal nicking enzyme amplification reaction and subsequent hybridization of the amplification product with gold nanoparticles and magnetic microparticles (barcode system) to achieve naked-eye colorimetric detection. This platform was used to detect a specific miRNA (miRNA-10b) associated with breast cancer, and attomolar sensitivity was demonstrated. The assay was validated in cell culture lysates from breast cancer cells and in serum from a mouse model of breast cancer.",
author = "Stefano Persano and Guevara, {Maria L.} and Joy Wolfram and Elvin Blanco and Haifa Shen and Mauro Ferrari and Pompa, {Pier Paolo}",
note = "Funding Information: This work was funded by the Houston Methodist Research Institute. Partial funds were acquired from the Ernest Cockrell Jr. Distinguished Endowed Chair (M.F.), the U.S. Department of Defense (W81XWH-09-1-0212, W81XWH-12-1-0414) (M.F.), the National Institutes of Health (U54CA143837, U54CA151668) (M.F.), the Cancer Prevention Research Institute of Texas (RP121071) (M.F. and H.S.), and the Italian Flagship Project NanoMax (S.P. and P.P.P.). Publisher Copyright: {\textcopyright} 2016 American Chemical Society.",
year = "2016",
month = sep,
day = "30",
doi = "10.1021/acsomega.6b00109",
language = "English (US)",
volume = "1",
pages = "448--455",
journal = "ACS Omega",
issn = "2470-1343",
publisher = "American Chemical Society",
number = "3",
}