Label-Free Isothermal Amplification Assay for Specific and Highly Sensitive Colorimetric miRNA Detection

Stefano Persano, Maria L. Guevara, Joy Wolfram, Elvin Blanco, Haifa Shen, Mauro Ferrari, Pier Paolo Pompa

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

We describe a new method for the detection of miRNA in biological samples. This technology is based on the isothermal nicking enzyme amplification reaction and subsequent hybridization of the amplification product with gold nanoparticles and magnetic microparticles (barcode system) to achieve naked-eye colorimetric detection. This platform was used to detect a specific miRNA (miRNA-10b) associated with breast cancer, and attomolar sensitivity was demonstrated. The assay was validated in cell culture lysates from breast cancer cells and in serum from a mouse model of breast cancer.

Original languageEnglish (US)
Pages (from-to)448-455
Number of pages8
JournalACS Omega
Volume1
Issue number3
DOIs
StatePublished - Sep 30 2016

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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