Label-Free Isothermal Amplification Assay for Specific and Highly Sensitive Colorimetric miRNA Detection

Stefano Persano; Maria L. Guevara; Joy Wolfram; Elvin Blanco; Haifa Shen; Mauro Ferrari; Pier Paolo Pompa

doi:10.1021/acsomega.6b00109

Label-Free Isothermal Amplification Assay for Specific and Highly Sensitive Colorimetric miRNA Detection

Stefano Persano, Maria L. Guevara, Joy Wolfram, Elvin Blanco, Haifa Shen, Mauro Ferrari, Pier Paolo Pompa

Transplant Medicine (Transplantation)

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

We describe a new method for the detection of miRNA in biological samples. This technology is based on the isothermal nicking enzyme amplification reaction and subsequent hybridization of the amplification product with gold nanoparticles and magnetic microparticles (barcode system) to achieve naked-eye colorimetric detection. This platform was used to detect a specific miRNA (miRNA-10b) associated with breast cancer, and attomolar sensitivity was demonstrated. The assay was validated in cell culture lysates from breast cancer cells and in serum from a mouse model of breast cancer.

Original language	English (US)
Pages (from-to)	448-455
Number of pages	8
Journal	ACS Omega
Volume	1
Issue number	3
DOIs	https://doi.org/10.1021/acsomega.6b00109
State	Published - Sep 30 2016

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)

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title = "Label-Free Isothermal Amplification Assay for Specific and Highly Sensitive Colorimetric miRNA Detection",

abstract = "We describe a new method for the detection of miRNA in biological samples. This technology is based on the isothermal nicking enzyme amplification reaction and subsequent hybridization of the amplification product with gold nanoparticles and magnetic microparticles (barcode system) to achieve naked-eye colorimetric detection. This platform was used to detect a specific miRNA (miRNA-10b) associated with breast cancer, and attomolar sensitivity was demonstrated. The assay was validated in cell culture lysates from breast cancer cells and in serum from a mouse model of breast cancer.",

author = "Stefano Persano and Guevara, {Maria L.} and Joy Wolfram and Elvin Blanco and Haifa Shen and Mauro Ferrari and Pompa, {Pier Paolo}",

note = "Funding Information: This work was funded by the Houston Methodist Research Institute. Partial funds were acquired from the Ernest Cockrell Jr. Distinguished Endowed Chair (M.F.), the U.S. Department of Defense (W81XWH-09-1-0212, W81XWH-12-1-0414) (M.F.), the National Institutes of Health (U54CA143837, U54CA151668) (M.F.), the Cancer Prevention Research Institute of Texas (RP121071) (M.F. and H.S.), and the Italian Flagship Project NanoMax (S.P. and P.P.P.).",

year = "2016",

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doi = "10.1021/acsomega.6b00109",

language = "English (US)",

volume = "1",

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T1 - Label-Free Isothermal Amplification Assay for Specific and Highly Sensitive Colorimetric miRNA Detection

AU - Persano, Stefano

AU - Guevara, Maria L.

AU - Wolfram, Joy

AU - Blanco, Elvin

AU - Shen, Haifa

AU - Ferrari, Mauro

AU - Pompa, Pier Paolo

N1 - Funding Information: This work was funded by the Houston Methodist Research Institute. Partial funds were acquired from the Ernest Cockrell Jr. Distinguished Endowed Chair (M.F.), the U.S. Department of Defense (W81XWH-09-1-0212, W81XWH-12-1-0414) (M.F.), the National Institutes of Health (U54CA143837, U54CA151668) (M.F.), the Cancer Prevention Research Institute of Texas (RP121071) (M.F. and H.S.), and the Italian Flagship Project NanoMax (S.P. and P.P.P.).

PY - 2016/9/30

Y1 - 2016/9/30

N2 - We describe a new method for the detection of miRNA in biological samples. This technology is based on the isothermal nicking enzyme amplification reaction and subsequent hybridization of the amplification product with gold nanoparticles and magnetic microparticles (barcode system) to achieve naked-eye colorimetric detection. This platform was used to detect a specific miRNA (miRNA-10b) associated with breast cancer, and attomolar sensitivity was demonstrated. The assay was validated in cell culture lysates from breast cancer cells and in serum from a mouse model of breast cancer.

AB - We describe a new method for the detection of miRNA in biological samples. This technology is based on the isothermal nicking enzyme amplification reaction and subsequent hybridization of the amplification product with gold nanoparticles and magnetic microparticles (barcode system) to achieve naked-eye colorimetric detection. This platform was used to detect a specific miRNA (miRNA-10b) associated with breast cancer, and attomolar sensitivity was demonstrated. The assay was validated in cell culture lysates from breast cancer cells and in serum from a mouse model of breast cancer.

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