Label-Free Assessment of Collagenase Digestion on Bovine Pericardium Properties by Fluorescence Lifetime Imaging

Cai Li, Jeny Shklover, Mojtaba Parvizi, Benjamin E. Sherlock, Alba Alfonso Garcia, Anne K. Haudenschild, Leigh G. Griffiths, Laura Marcu

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The extracellular matrix architecture of bovine pericardium (BP) has distinct biochemical and biomechanical properties that make it a useful biomaterial in the field of regenerative medicine. Collagen represents the dominant structural protein of BP and is therefore intimately associated with the properties of this biomaterial. Enzymatic degradation of collagen molecules is critical for extracellular matrix turnover, remodeling and ultimately tissue regeneration. We present a quantitative, label-free and non-destructive method for monitoring changes in biochemical and biomechanical properties of BP during tissue degradation, based on multi-spectral fluorescence lifetime imaging (ms-FLIm). Strong correlations of fluorescence intensity ratio and average fluorescence lifetime were identified with collagen content, Young’s Modulus and Ultimate tensile strength during collagenase degradation, indicating the potential of optically monitoring collagen degradation using ms-FLIm. The obtained results demonstrate the value of ms-FLIm to assess the quality of biomaterials in situ for applications in regenerative medicine.

Original languageEnglish (US)
Pages (from-to)1870-1881
Number of pages12
JournalAnnals of Biomedical Engineering
Issue number11
StatePublished - Nov 15 2018


  • Collagenase degradation
  • Fluorescence lifetime imaging
  • Non-destructive monitoring

ASJC Scopus subject areas

  • Biomedical Engineering


Dive into the research topics of 'Label-Free Assessment of Collagenase Digestion on Bovine Pericardium Properties by Fluorescence Lifetime Imaging'. Together they form a unique fingerprint.

Cite this