KRAS and BRAF mutation analysis from liquid-based cytology brushings of colorectal carcinoma in comparison with formalin-fixed, paraffin-embedded tissue

Aris Spathis, John Georgoulakis, Periklis Foukas, Maria Kefala, Konstantinos Leventakos, Anastasios Machairas, Ioannis Panayiotides, Petros Karakitsos

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Real-time PCR-based molecular diagnostic techniques were used for the comparative identification of the most common KRAS and BRAF mutations from methanol liquid-based cytology samples and matched, formalin-fixed and paraffin-embedded tissue samples. Methanol-based fixation and preservation of brushing samples of colorectal carcinomas could contribute to sample collection, short-term storage and subsequent molecular analysis, by improving workflow and enhancing the identification of putative mutations.

Original languageEnglish (US)
Pages (from-to)1969-1975
Number of pages7
JournalAnticancer research
Volume30
Issue number6
StatePublished - Jun 1 2010

Keywords

  • BRAF
  • Colorectal
  • FFPE
  • KRAS
  • Mutations
  • ThinPrep
  • qPCR

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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