KRAS and BRAF mutation analysis from liquid-based cytology brushings of colorectal carcinoma in comparison with formalin-fixed, paraffin-embedded tissue

Aris Spathis, John Georgoulakis, Periklis Foukas, Maria Kefala, Konstantinos Leventakos, Anastasios Machairas, Ioannis Panayiotides, Petros Karakitsos

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Real-time PCR-based molecular diagnostic techniques were used for the comparative identification of the most common KRAS and BRAF mutations from methanol liquid-based cytology samples and matched, formalin-fixed and paraffin-embedded tissue samples. Methanol-based fixation and preservation of brushing samples of colorectal carcinomas could contribute to sample collection, short-term storage and subsequent molecular analysis, by improving workflow and enhancing the identification of putative mutations.

Original languageEnglish (US)
Pages (from-to)1969-1975
Number of pages7
JournalAnticancer Research
Volume30
Issue number6
StatePublished - Jun 1 2010
Externally publishedYes

Fingerprint

Paraffin
Formaldehyde
Methanol
Cell Biology
Colorectal Neoplasms
Molecular Diagnostic Techniques
Mutation
Workflow
Real-Time Polymerase Chain Reaction

Keywords

  • BRAF
  • Colorectal
  • FFPE
  • KRAS
  • Mutations
  • qPCR
  • ThinPrep

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

KRAS and BRAF mutation analysis from liquid-based cytology brushings of colorectal carcinoma in comparison with formalin-fixed, paraffin-embedded tissue. / Spathis, Aris; Georgoulakis, John; Foukas, Periklis; Kefala, Maria; Leventakos, Konstantinos; Machairas, Anastasios; Panayiotides, Ioannis; Karakitsos, Petros.

In: Anticancer Research, Vol. 30, No. 6, 01.06.2010, p. 1969-1975.

Research output: Contribution to journalArticle

Spathis, A, Georgoulakis, J, Foukas, P, Kefala, M, Leventakos, K, Machairas, A, Panayiotides, I & Karakitsos, P 2010, 'KRAS and BRAF mutation analysis from liquid-based cytology brushings of colorectal carcinoma in comparison with formalin-fixed, paraffin-embedded tissue', Anticancer Research, vol. 30, no. 6, pp. 1969-1975.
Spathis, Aris ; Georgoulakis, John ; Foukas, Periklis ; Kefala, Maria ; Leventakos, Konstantinos ; Machairas, Anastasios ; Panayiotides, Ioannis ; Karakitsos, Petros. / KRAS and BRAF mutation analysis from liquid-based cytology brushings of colorectal carcinoma in comparison with formalin-fixed, paraffin-embedded tissue. In: Anticancer Research. 2010 ; Vol. 30, No. 6. pp. 1969-1975.
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