Krüppel-like factor KLF10 is a link between the circadian clock and metabolism in liver

Fabienne Guillaumond, Aline Gréchez-Cassiau, Malayannan Subramaniam, Sophie Brangolo, Brigitta Peteri-Brünback, Bart Staels, Catherine Fiévet, Thomas C. Spelsberg, Franck Delaunay, Michèle Teboul

Research output: Contribution to journalArticle

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Abstract

The circadian timing system coordinates many aspects of mammalian physiology and behavior in synchrony with the external light/dark cycle. These rhythms are driven by endogenous molecular clocks present in most body cells. Many clock outputs are transcriptional regulators, suggesting that clock genes primarily control physiology through indirect pathways. Here, we show that Krüppel-like factor 10 (KLF10) displays a robust circadian expression pattern in wild-type mouse liver but not in clock-deficient Bmal1 knockout mice. Consistently, the Klf10 promoter recruited the BMAL1 core clock protein and was transactivated by the CLOCK-BMAL1 heterodimer through a conserved E-box response element. Profiling the liver transcriptome from Klf10-/- mice identified 158 regulated genes with significant enrichment for transcripts involved in lipid and carbohydrate metabolism. Importantly, approximately 56% of these metabolic genes are clock controlled. Male Klf10-/- mice displayed postprandial and fasting hyperglycemia, a phenotype accompanied by a significant time-of-day-dependent upregulation of the gluconeogenic gene Pepck and increased hepatic glucose production. Consistently, functional data showed that the proximal Pepck promoter is repressed directly by KLF10. Klf10 -/- females were normoglycemic but displayed higher plasma triglycerides. Correspondingly, rhythmic gene expression of components of the lipogenic pathway, including Srebp1c, Fas, and Elovl6, was altered in females. Collectively, these data establish KLF10 as a required circadian transcriptional regulator that links the molecular clock to energy metabolism in the liver.

Original languageEnglish (US)
Pages (from-to)3059-3070
Number of pages12
JournalMolecular and Cellular Biology
Volume30
Issue number12
DOIs
StatePublished - Jun 2010

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Circadian Clocks
Liver
Genes
E-Box Elements
Gene Components
Photoperiod
Carbohydrate Metabolism
Response Elements
Gene Expression Profiling
Lipid Metabolism
Knockout Mice
Hyperglycemia
Energy Metabolism
Fasting
Triglycerides
Up-Regulation
Phenotype
Gene Expression
Glucose
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Guillaumond, F., Gréchez-Cassiau, A., Subramaniam, M., Brangolo, S., Peteri-Brünback, B., Staels, B., ... Teboul, M. (2010). Krüppel-like factor KLF10 is a link between the circadian clock and metabolism in liver. Molecular and Cellular Biology, 30(12), 3059-3070. https://doi.org/10.1128/MCB.01141-09

Krüppel-like factor KLF10 is a link between the circadian clock and metabolism in liver. / Guillaumond, Fabienne; Gréchez-Cassiau, Aline; Subramaniam, Malayannan; Brangolo, Sophie; Peteri-Brünback, Brigitta; Staels, Bart; Fiévet, Catherine; Spelsberg, Thomas C.; Delaunay, Franck; Teboul, Michèle.

In: Molecular and Cellular Biology, Vol. 30, No. 12, 06.2010, p. 3059-3070.

Research output: Contribution to journalArticle

Guillaumond, F, Gréchez-Cassiau, A, Subramaniam, M, Brangolo, S, Peteri-Brünback, B, Staels, B, Fiévet, C, Spelsberg, TC, Delaunay, F & Teboul, M 2010, 'Krüppel-like factor KLF10 is a link between the circadian clock and metabolism in liver', Molecular and Cellular Biology, vol. 30, no. 12, pp. 3059-3070. https://doi.org/10.1128/MCB.01141-09
Guillaumond F, Gréchez-Cassiau A, Subramaniam M, Brangolo S, Peteri-Brünback B, Staels B et al. Krüppel-like factor KLF10 is a link between the circadian clock and metabolism in liver. Molecular and Cellular Biology. 2010 Jun;30(12):3059-3070. https://doi.org/10.1128/MCB.01141-09
Guillaumond, Fabienne ; Gréchez-Cassiau, Aline ; Subramaniam, Malayannan ; Brangolo, Sophie ; Peteri-Brünback, Brigitta ; Staels, Bart ; Fiévet, Catherine ; Spelsberg, Thomas C. ; Delaunay, Franck ; Teboul, Michèle. / Krüppel-like factor KLF10 is a link between the circadian clock and metabolism in liver. In: Molecular and Cellular Biology. 2010 ; Vol. 30, No. 12. pp. 3059-3070.
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