Krüppel-Like Factor 10 participates in cervical cancer immunoediting through transcriptional regulation of Pregnancy-Specific Beta-1 Glycoproteins

Daniel Marrero-Rodríguez, Keiko Taniguchi-Ponciano, Malayannan Subramaniam, John R Hawse, Kevin S. Pitel, Hugo Arreola-De La Cruz, Victor Huerta-Padilla, Gustavo Ponce-Navarrete, Ma Del Pilar Figueroa-Corona, Laura Gomez-Virgilio, Teresa I. Martinez-Cuevas, Monica Mendoza-Rodriguez, Miriam Rodriguez-Esquivel, Pablo Romero-Morelos, Jorge Ramirez-Salcedo, Michael Baudis, Marco Meraz-Rios, Florinda Jimenez-Vega, Mauricio Salcedo

Research output: Contribution to journalArticle

Abstract

Cervical cancer (CC) is associated with alterations in immune system balance, which is primarily due to a shift from Th1 to Th2 and the unbalance of Th17/Treg cells. Using in silico DNA copy number analysis, we have demonstrated that ~20% of CC samples exhibit gain of 8q22.3 and 19q13.31; the regions of the genome that encodes the KLF10 and PSG genes, respectively. Gene expression studies demonstrated that there were no alterations in KLF10 mRNA expression, whilst the PSG2 and -5 genes were up-regulated by 1.76 and 3.97-fold respectively in CC compared to normal tissue controls. siRNA and ChIP experiments in SiHa cells have demonstrated that KLF10 participates in immune response through regulation of IL6, IL25 and PSG2 and PSG5 genes. Using cervical tissues from KLF10-/- mice, we have identified down-regulation of PSG17, -21 and -23 and IL11. These results suggest that KLF10 may regulate immune system response genes in cervical cancer among other functions. KLF10 and PSG copy number variations and alterations in mRNA expression levels could represent novel molecular markers in CC.

Original languageEnglish (US)
Article number9445
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Pregnancy-Specific beta 1-Glycoproteins
Uterine Cervical Neoplasms
Genes
Immune System
Interleukin-11
Th17 Cells
Messenger RNA
Regulatory T-Lymphocytes
Computer Simulation
Small Interfering RNA
Interleukin-6
Down-Regulation
Genome
Gene Expression
DNA

ASJC Scopus subject areas

  • General

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Krüppel-Like Factor 10 participates in cervical cancer immunoediting through transcriptional regulation of Pregnancy-Specific Beta-1 Glycoproteins. / Marrero-Rodríguez, Daniel; Taniguchi-Ponciano, Keiko; Subramaniam, Malayannan; Hawse, John R; Pitel, Kevin S.; Arreola-De La Cruz, Hugo; Huerta-Padilla, Victor; Ponce-Navarrete, Gustavo; Figueroa-Corona, Ma Del Pilar; Gomez-Virgilio, Laura; Martinez-Cuevas, Teresa I.; Mendoza-Rodriguez, Monica; Rodriguez-Esquivel, Miriam; Romero-Morelos, Pablo; Ramirez-Salcedo, Jorge; Baudis, Michael; Meraz-Rios, Marco; Jimenez-Vega, Florinda; Salcedo, Mauricio.

In: Scientific Reports, Vol. 8, No. 1, 9445, 01.12.2018.

Research output: Contribution to journalArticle

Marrero-Rodríguez, D, Taniguchi-Ponciano, K, Subramaniam, M, Hawse, JR, Pitel, KS, Arreola-De La Cruz, H, Huerta-Padilla, V, Ponce-Navarrete, G, Figueroa-Corona, MDP, Gomez-Virgilio, L, Martinez-Cuevas, TI, Mendoza-Rodriguez, M, Rodriguez-Esquivel, M, Romero-Morelos, P, Ramirez-Salcedo, J, Baudis, M, Meraz-Rios, M, Jimenez-Vega, F & Salcedo, M 2018, 'Krüppel-Like Factor 10 participates in cervical cancer immunoediting through transcriptional regulation of Pregnancy-Specific Beta-1 Glycoproteins', Scientific Reports, vol. 8, no. 1, 9445. https://doi.org/10.1038/s41598-018-27711-8
Marrero-Rodríguez, Daniel ; Taniguchi-Ponciano, Keiko ; Subramaniam, Malayannan ; Hawse, John R ; Pitel, Kevin S. ; Arreola-De La Cruz, Hugo ; Huerta-Padilla, Victor ; Ponce-Navarrete, Gustavo ; Figueroa-Corona, Ma Del Pilar ; Gomez-Virgilio, Laura ; Martinez-Cuevas, Teresa I. ; Mendoza-Rodriguez, Monica ; Rodriguez-Esquivel, Miriam ; Romero-Morelos, Pablo ; Ramirez-Salcedo, Jorge ; Baudis, Michael ; Meraz-Rios, Marco ; Jimenez-Vega, Florinda ; Salcedo, Mauricio. / Krüppel-Like Factor 10 participates in cervical cancer immunoediting through transcriptional regulation of Pregnancy-Specific Beta-1 Glycoproteins. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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AU - Subramaniam, Malayannan

AU - Hawse, John R

AU - Pitel, Kevin S.

AU - Arreola-De La Cruz, Hugo

AU - Huerta-Padilla, Victor

AU - Ponce-Navarrete, Gustavo

AU - Figueroa-Corona, Ma Del Pilar

AU - Gomez-Virgilio, Laura

AU - Martinez-Cuevas, Teresa I.

AU - Mendoza-Rodriguez, Monica

AU - Rodriguez-Esquivel, Miriam

AU - Romero-Morelos, Pablo

AU - Ramirez-Salcedo, Jorge

AU - Baudis, Michael

AU - Meraz-Rios, Marco

AU - Jimenez-Vega, Florinda

AU - Salcedo, Mauricio

PY - 2018/12/1

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N2 - Cervical cancer (CC) is associated with alterations in immune system balance, which is primarily due to a shift from Th1 to Th2 and the unbalance of Th17/Treg cells. Using in silico DNA copy number analysis, we have demonstrated that ~20% of CC samples exhibit gain of 8q22.3 and 19q13.31; the regions of the genome that encodes the KLF10 and PSG genes, respectively. Gene expression studies demonstrated that there were no alterations in KLF10 mRNA expression, whilst the PSG2 and -5 genes were up-regulated by 1.76 and 3.97-fold respectively in CC compared to normal tissue controls. siRNA and ChIP experiments in SiHa cells have demonstrated that KLF10 participates in immune response through regulation of IL6, IL25 and PSG2 and PSG5 genes. Using cervical tissues from KLF10-/- mice, we have identified down-regulation of PSG17, -21 and -23 and IL11. These results suggest that KLF10 may regulate immune system response genes in cervical cancer among other functions. KLF10 and PSG copy number variations and alterations in mRNA expression levels could represent novel molecular markers in CC.

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