Knockout of CD8 Delays Reendothelialization and Accelerates Neointima Formation in Injured Arteries of Mouse via TNF-α Inhibiting the Endothelial Cells Migration

Jun Meng Zhang, Ying Wang, Yan Ju Miao, Yi Zhang, Yi Na Wu, Li Xin Jia, Yong Fen Qi, Jie Du

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Objective:Delayed or impaired reendothelialization is a major cause of stent thrombosis in the interventional treatment of coronary heart disease. T cells are involved in neointima formation of injured arteries. However, the regulated mechanism of reendothelialization and the role of CD8 T cell in reendothelialization are unclear.Methods and Results:Immunofluorescence staining showed that CD8 positive cells were increased in wire injured femoral artery of mice. On day 21 after injury, elastin staining showed that knockout of CD8 (CD8-/-) significantly increased intimal thickness and a ratio of intima to media by 1.8 folds and 1.9 folds respectively in injured arteries. Evans blue staining showed that knockout of CD8 delayed the reendothelialization area on day 7 after injury (18.8±0.5% versus 42.1±5.6%, p<0.05). In vitro, a migration assay revealed that CD8-/- T cells co-cultured with WT macrophages significantly inhibited the migration of the endothelial cells (ECs); compared to CD4+ T cells, and CD8+ T cells could promote the ECs migration. Furthermore, real-time PCR analysis showed that knockout of CD8 increased the level of tumor necrosis factor α (TNF-α) in injured arteries and cytometric bead cytokine array showed that TNF-α was elevated in cultured CD8-/- T cells. Finally, a wound-healing assay showed that recombinant TNF-α significantly inhibited the migration of ECs.Conclusion:Our study suggested that CD8+ T cells could promote the reendothelialization and inhibit the neointima formation after the artery wire injury, and this effect is at least partly dependent on decreasing TNF-α production promoting ECs migration.

Original languageEnglish (US)
Article numbere62001
JournalPloS one
Volume8
Issue number5
DOIs
StatePublished - May 2 2013

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Knockout of CD8 Delays Reendothelialization and Accelerates Neointima Formation in Injured Arteries of Mouse via TNF-α Inhibiting the Endothelial Cells Migration'. Together they form a unique fingerprint.

Cite this