KLF6 degradation after apoptotic DNA damage

Michaela S. Banck, Simon W. Beaven, Goutham Narla, Martin J. Walsh, Scott L. Friedman, Andreas S Beutler

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Krüppel-like factor 6 (KLF6) is a cancer gene (www.sanger.ac.uk/genetics/CGP/Census/). Here, we demonstrate that KLF6 protein is rapidly degraded when apoptosis is induced via the intrinsic pathway by cisplatin, adriamycin, or UVB irradiation in multiple cell lines (HCT116, SW40, HepG2, PC3-M, Skov3, NIH-3T3, 293T, GM09706, and MEF, IMR-90). KLF6 degradation occurred in the presence or absence of p53, was associated with ubiquitination, mediated by the proteasome (half-life 16 min, unstimulated), and independent of caspases and calpain. KLF6 was unchanged by apoptosis via the extrinsic/death-receptor pathway. Deregulation of KLF6 stability may alter its tumor suppressor function and/or the response of tumors to chemotherapeutics.

Original languageEnglish (US)
Pages (from-to)6981-6986
Number of pages6
JournalFEBS Letters
Volume580
Issue number30
DOIs
StatePublished - Dec 22 2006
Externally publishedYes

Fingerprint

DNA Damage
Tumors
Apoptosis
Degradation
Death Domain Receptors
Calpain
Deregulation
Neoplasm Genes
Ubiquitination
DNA
Censuses
Proteasome Endopeptidase Complex
Caspases
Doxorubicin
Cisplatin
Half-Life
Neoplasms
Genes
Cells
Irradiation

Keywords

  • Apoptosis
  • Cancer cells
  • Carcinogenesis
  • Chemosensitivity
  • DNA-damage
  • KLF6
  • Krüppel-like factor
  • Proteasomal degradation
  • Ubiquitin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Banck, M. S., Beaven, S. W., Narla, G., Walsh, M. J., Friedman, S. L., & Beutler, A. S. (2006). KLF6 degradation after apoptotic DNA damage. FEBS Letters, 580(30), 6981-6986. https://doi.org/10.1016/j.febslet.2006.10.077

KLF6 degradation after apoptotic DNA damage. / Banck, Michaela S.; Beaven, Simon W.; Narla, Goutham; Walsh, Martin J.; Friedman, Scott L.; Beutler, Andreas S.

In: FEBS Letters, Vol. 580, No. 30, 22.12.2006, p. 6981-6986.

Research output: Contribution to journalArticle

Banck, MS, Beaven, SW, Narla, G, Walsh, MJ, Friedman, SL & Beutler, AS 2006, 'KLF6 degradation after apoptotic DNA damage', FEBS Letters, vol. 580, no. 30, pp. 6981-6986. https://doi.org/10.1016/j.febslet.2006.10.077
Banck MS, Beaven SW, Narla G, Walsh MJ, Friedman SL, Beutler AS. KLF6 degradation after apoptotic DNA damage. FEBS Letters. 2006 Dec 22;580(30):6981-6986. https://doi.org/10.1016/j.febslet.2006.10.077
Banck, Michaela S. ; Beaven, Simon W. ; Narla, Goutham ; Walsh, Martin J. ; Friedman, Scott L. ; Beutler, Andreas S. / KLF6 degradation after apoptotic DNA damage. In: FEBS Letters. 2006 ; Vol. 580, No. 30. pp. 6981-6986.
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