Kit signaling is required for development of coordinated motility patterns in zebrafish gastrointestinal tract

Adam Rich, Scott Gordon, Chris Brown, Simon J. Gibbons, Katherine Schaefer, Grant Hennig, Gianrico Farrugia

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Interstitial cells of Cajal (ICC) provide a pacemaker signal for coordinated motility patterns in the mammalian gastrointestinal (GI) tract. Kit signaling is required for development and maintenance of ICC, and these cells can be identified by Kit-like immunoreactivity. The zebrafish GI tract has two distinct ICC networks similar to mammals, suggesting a similar role in the generation of GI motility; however, a functional role for Kit-positive cells in zebrafish has not been determined. Analysis of GI motility in intact zebrafish larvae was performed during development and after disruption of Kit signaling. Development of coordinated motility patterns occurred after 5 days post-fertilization (dpf) and correlated with appearance of Kit-positive cells. Disruptions of Kit signaling using the Kit antagonist imatinib mesylate, and in Sparse, a null kita mutant, also disrupted development of coordinated motility patterns. These data suggest that Kit signaling is necessary for development of coordinated motility patterns and that Kit-positive cells in zebrafish are necessary for coordinated motility patterns.

Original languageEnglish (US)
Pages (from-to)154-160
Number of pages7
JournalZebrafish
Volume10
Issue number2
DOIs
StatePublished - Jun 1 2013

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology

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