Kinetics of [18F]1-fluoro-2,4-dinitrobenzene, a potential probe for the glutathione detoxification system, in perfused working rat heart

M. L. Kornguth, J. E. Holden, Timothy R DeGrado, C. K. Ng, D. M. Raffel, S. J. Gatley

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

[18F]1-Fluoro-2,4-dinitrobenzene (FDNB), a substrate for the detoxification enzyme glutathione-S-transferase, was prepared by 18F-Cl exchange. It was administered as a rapid bolus in the perfused working rat heart and the kinetics of the label were followed by external coincidence detection and by analysis of the coronary effluents and tissue homogenates. The data suggest that part of the extracted tracer was released, part was dehalogenated and part was reversibly incorporated into a large tissue pool of FDNB that was not a substrate for defluorination. The rates of the specific processes, estimated from the residue data, may be sensitive to changes in the glutathione detoxification system and therefore applicable to investigation of the compromised myocardium.

Original languageEnglish (US)
Pages (from-to)519-524
Number of pages6
JournalNuclear Medicine and Biology
Volume16
Issue number5
StatePublished - 1989
Externally publishedYes

Fingerprint

Dinitrofluorobenzene
Glutathione
Glutathione Transferase
Myocardium
Enzymes
2,4-dinitrobenzene

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Medicine(all)

Cite this

Kinetics of [18F]1-fluoro-2,4-dinitrobenzene, a potential probe for the glutathione detoxification system, in perfused working rat heart. / Kornguth, M. L.; Holden, J. E.; DeGrado, Timothy R; Ng, C. K.; Raffel, D. M.; Gatley, S. J.

In: Nuclear Medicine and Biology, Vol. 16, No. 5, 1989, p. 519-524.

Research output: Contribution to journalArticle

@article{fb449cde392e416fa969533444ad2b72,
title = "Kinetics of [18F]1-fluoro-2,4-dinitrobenzene, a potential probe for the glutathione detoxification system, in perfused working rat heart",
abstract = "[18F]1-Fluoro-2,4-dinitrobenzene (FDNB), a substrate for the detoxification enzyme glutathione-S-transferase, was prepared by 18F-Cl exchange. It was administered as a rapid bolus in the perfused working rat heart and the kinetics of the label were followed by external coincidence detection and by analysis of the coronary effluents and tissue homogenates. The data suggest that part of the extracted tracer was released, part was dehalogenated and part was reversibly incorporated into a large tissue pool of FDNB that was not a substrate for defluorination. The rates of the specific processes, estimated from the residue data, may be sensitive to changes in the glutathione detoxification system and therefore applicable to investigation of the compromised myocardium.",
author = "Kornguth, {M. L.} and Holden, {J. E.} and DeGrado, {Timothy R} and Ng, {C. K.} and Raffel, {D. M.} and Gatley, {S. J.}",
year = "1989",
language = "English (US)",
volume = "16",
pages = "519--524",
journal = "International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology",
issn = "0969-8051",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - Kinetics of [18F]1-fluoro-2,4-dinitrobenzene, a potential probe for the glutathione detoxification system, in perfused working rat heart

AU - Kornguth, M. L.

AU - Holden, J. E.

AU - DeGrado, Timothy R

AU - Ng, C. K.

AU - Raffel, D. M.

AU - Gatley, S. J.

PY - 1989

Y1 - 1989

N2 - [18F]1-Fluoro-2,4-dinitrobenzene (FDNB), a substrate for the detoxification enzyme glutathione-S-transferase, was prepared by 18F-Cl exchange. It was administered as a rapid bolus in the perfused working rat heart and the kinetics of the label were followed by external coincidence detection and by analysis of the coronary effluents and tissue homogenates. The data suggest that part of the extracted tracer was released, part was dehalogenated and part was reversibly incorporated into a large tissue pool of FDNB that was not a substrate for defluorination. The rates of the specific processes, estimated from the residue data, may be sensitive to changes in the glutathione detoxification system and therefore applicable to investigation of the compromised myocardium.

AB - [18F]1-Fluoro-2,4-dinitrobenzene (FDNB), a substrate for the detoxification enzyme glutathione-S-transferase, was prepared by 18F-Cl exchange. It was administered as a rapid bolus in the perfused working rat heart and the kinetics of the label were followed by external coincidence detection and by analysis of the coronary effluents and tissue homogenates. The data suggest that part of the extracted tracer was released, part was dehalogenated and part was reversibly incorporated into a large tissue pool of FDNB that was not a substrate for defluorination. The rates of the specific processes, estimated from the residue data, may be sensitive to changes in the glutathione detoxification system and therefore applicable to investigation of the compromised myocardium.

UR - http://www.scopus.com/inward/record.url?scp=0024339852&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024339852&partnerID=8YFLogxK

M3 - Article

VL - 16

SP - 519

EP - 524

JO - International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology

JF - International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology

SN - 0969-8051

IS - 5

ER -