Kinetic effects of terbium ions on muscarinic acetylcholine receptors of murine neuroblastoma cells

E. E. El-Fakahany, M. Pfenning, E. Richelson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Preincubation of murine neuroblastoma cells (clone N1E-115) with terbium chloride resulted in a significant potentiation of carbachol-mediated increase in cyclic GMP formation. This effect was accompanied by a shift of the peak response from 30 s to 120 s and a 6-fold decrease in carbachol concentration producing half-maximal responses, in addition to a significant increase in the Hill coefficient. Terbium ions also caused a significant decrease in the affinity and an increase in the maximum binding of [3H]quinuclidinyl benzilate to muscarinic receptors, the change in affinity being mainly due to a decrease in the association rate. Preincubation of cells with 1 mM carbachol for 4 h (the desensitized state of the muscarinic receptor) resulted in a decrease in the ability of terbium to alter [3H]quinuclidinyl benzilate binding. The effects of terbium reported here might be due to its affecting muscarinic receptor-effector coupling, which is considered to be lost upon receptor desensitization.

Original languageEnglish (US)
Pages (from-to)863-869
Number of pages7
JournalJournal of Neurochemistry
Volume42
Issue number3
StatePublished - 1984

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Terbium
Carbachol
Muscarinic Receptors
Neuroblastoma
Quinuclidinyl Benzilate
Ions
Kinetics
Clone cells
Cyclic GMP
Clone Cells

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Kinetic effects of terbium ions on muscarinic acetylcholine receptors of murine neuroblastoma cells. / El-Fakahany, E. E.; Pfenning, M.; Richelson, E.

In: Journal of Neurochemistry, Vol. 42, No. 3, 1984, p. 863-869.

Research output: Contribution to journalArticle

El-Fakahany, EE, Pfenning, M & Richelson, E 1984, 'Kinetic effects of terbium ions on muscarinic acetylcholine receptors of murine neuroblastoma cells', Journal of Neurochemistry, vol. 42, no. 3, pp. 863-869.
El-Fakahany, E. E. ; Pfenning, M. ; Richelson, E. / Kinetic effects of terbium ions on muscarinic acetylcholine receptors of murine neuroblastoma cells. In: Journal of Neurochemistry. 1984 ; Vol. 42, No. 3. pp. 863-869.
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