Kinetic control in protein folding for light chain amyloidosis and the differential effects of somatic mutations

Luis M. Blancas-Mejía, Alexander Tischer, James R. Thompson, Jonathan Tai, Lin Wang, Matthew Auton, Marina Ramirez-Alvarado

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Light chain amyloidosis is a devastating disease where immunoglobulin light chains form amyloid fibrils, resulting in organ dysfunction and death. Previous studies have shown a direct correlation between the protein thermodynamic stability and the propensity for amyloid formation for some proteins involved in light chain amyloidosis. Here we investigate the effect of somatic mutations on protein stability and in vitro fibril formation of single and double restorative mutants of the protein AL-103 compared to the wild-type germline control protein. A scan rate dependence and hysteresis in the thermal unfolding and refolding was observed for all proteins. This indicates that the unfolding/refolding reaction is kinetically determined with different kinetic constants for unfolding and refolding even though the process remains experimentally reversible. Our structural analysis of AL-103 and AL-103 delP95aIns suggests a kinetic coupling of the unfolding/refolding process with cis-trans prolyl isomerization. Our data reveal that the deletion of proline 95a (AL-103 delP95aIns), which removes the trans-cis di-proline motif present in the patient protein AL-103, results in a dramatic increment in the thermodynamic stability and a significant delay in fibril formation kinetics with respect to AL-103. Fibril formation is pH dependent; all proteins form fibrils at pH 2; reactions become slower and more stochastic as the pH increases up to pH 7. Based on these results, we propose that, in addition to thermodynamic stability, kinetic stability (possibly influenced by the presence of cis proline 95a) plays a major role in the AL-103 amyloid fibril formation process.

Original languageEnglish (US)
Pages (from-to)347-361
Number of pages15
JournalJournal of Molecular Biology
Volume426
Issue number2
DOIs
StatePublished - Jan 23 2014

Keywords

  • amyloid fibril formation
  • kinetic two-state model
  • light chain amyloidosis
  • protein thermodynamic and kinetic stability
  • scan rate dependence and hysteresis

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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