@inbook{7dce9d332ea14186a6736a95f95c0e66,
title = "Kidney harvesting and metabolite extraction for metabolomics studies in rodents",
abstract = "Elucidating the metabolic changes that accompany disease states via metabolomics analysis of tissues has become an important avenue of exploration in biomarker and therapeutic target discovery. Conventional harvesting techniques rely on post-euthanasia tissue harvest which introduces ischemic conditions and subsequent metabolome changes that may ultimately introduce artifacts into final analyses. In this chapter, we present protocols for low-ischemia time rapid kidney tissue harvest followed by metabolite extraction for metabolomics studies in rodents.",
keywords = "Ischemia/reperfusion, Metabolomics, Tissue harvest",
author = "Igor Maksimovic and Song Zhang and Ivan Vuckovic and Irazabal, {Maria V.}",
note = "Funding Information: This work is supported by the National Institutes of Health grant DK118391 to M.V.I., the nuSURF Program (DK101405) and the Mayo Translational Polycystic Kidney Disease Center (NIDDK P30 DK090728). Funding Information: This work is supported by the National Institutes of Health grant DK118391 to M.V.I. the nuSURF Program (DK101405) and the Mayo Translational Polycystic Kidney Disease Center (NIDDK P30 DK090728). Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
doi = "10.1016/bs.mcb.2019.05.009",
language = "English (US)",
isbn = "9780128203354",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc",
pages = "15--29",
editor = "Thomas Weimbs",
booktitle = "Methods in Kidney Cell Biology - Part B",
}