Kidney harvesting and metabolite extraction for metabolomics studies in rodents

Igor Maksimovic, Song Zhang, Ivan Vuckovic, Maria Irazabal Mira

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Elucidating the metabolic changes that accompany disease states via metabolomics analysis of tissues has become an important avenue of exploration in biomarker and therapeutic target discovery. Conventional harvesting techniques rely on post-euthanasia tissue harvest which introduces ischemic conditions and subsequent metabolome changes that may ultimately introduce artifacts into final analyses. In this chapter, we present protocols for low-ischemia time rapid kidney tissue harvest followed by metabolite extraction for metabolomics studies in rodents.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Cell Biology
ISSN (Print)0091-679X

Fingerprint

Metabolomics
Rodentia
Kidney
Euthanasia
Metabolome
Artifacts
Ischemia
Biomarkers
Therapeutics

Keywords

  • Ischemia/reperfusion
  • Metabolomics
  • Tissue harvest

ASJC Scopus subject areas

  • Cell Biology

Cite this

Maksimovic, I., Zhang, S., Vuckovic, I., & Irazabal Mira, M. (2019). Kidney harvesting and metabolite extraction for metabolomics studies in rodents. In Methods in Cell Biology (Methods in Cell Biology). Academic Press Inc.. https://doi.org/10.1016/bs.mcb.2019.05.009

Kidney harvesting and metabolite extraction for metabolomics studies in rodents. / Maksimovic, Igor; Zhang, Song; Vuckovic, Ivan; Irazabal Mira, Maria.

Methods in Cell Biology. Academic Press Inc., 2019. (Methods in Cell Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter

Maksimovic, I, Zhang, S, Vuckovic, I & Irazabal Mira, M 2019, Kidney harvesting and metabolite extraction for metabolomics studies in rodents. in Methods in Cell Biology. Methods in Cell Biology, Academic Press Inc. https://doi.org/10.1016/bs.mcb.2019.05.009
Maksimovic I, Zhang S, Vuckovic I, Irazabal Mira M. Kidney harvesting and metabolite extraction for metabolomics studies in rodents. In Methods in Cell Biology. Academic Press Inc. 2019. (Methods in Cell Biology). https://doi.org/10.1016/bs.mcb.2019.05.009
Maksimovic, Igor ; Zhang, Song ; Vuckovic, Ivan ; Irazabal Mira, Maria. / Kidney harvesting and metabolite extraction for metabolomics studies in rodents. Methods in Cell Biology. Academic Press Inc., 2019. (Methods in Cell Biology).
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