Kidney harvesting and metabolite extraction for metabolomics studies in rodents

Igor Maksimovic, Song Zhang, Ivan Vuckovic, Maria V. Irazabal

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Elucidating the metabolic changes that accompany disease states via metabolomics analysis of tissues has become an important avenue of exploration in biomarker and therapeutic target discovery. Conventional harvesting techniques rely on post-euthanasia tissue harvest which introduces ischemic conditions and subsequent metabolome changes that may ultimately introduce artifacts into final analyses. In this chapter, we present protocols for low-ischemia time rapid kidney tissue harvest followed by metabolite extraction for metabolomics studies in rodents.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
EditorsThomas Weimbs
PublisherAcademic Press Inc
Pages15-29
Number of pages15
ISBN (Print)9780128203354
DOIs
StatePublished - 2019

Publication series

NameMethods in Cell Biology
Volume154
ISSN (Print)0091-679X

Keywords

  • Ischemia/reperfusion
  • Metabolomics
  • Tissue harvest

ASJC Scopus subject areas

  • Cell Biology

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  • Cite this

    Maksimovic, I., Zhang, S., Vuckovic, I., & Irazabal, M. V. (2019). Kidney harvesting and metabolite extraction for metabolomics studies in rodents. In T. Weimbs (Ed.), Methods in Cell Biology (pp. 15-29). (Methods in Cell Biology; Vol. 154). Academic Press Inc. https://doi.org/10.1016/bs.mcb.2019.05.009