Key role of alkaline phosphatase in the development of human-derived nanoparticles in vitro

Larry W. Hunter, Farooq A. Shiekh, George T. Pisimisis, Sung Hoon Kim, Samuel N. Edeh, Virginia M Miller, John C Lieske

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Alkaline phosphatase (ALP) is an enzyme critical for physiological and pathological biomineralization. Experiments were designed to determine whether ALP participates in the formation of calcifying nanometer sized particles (NPs) in vitro. Filtered homogenates of human calcified carotid artery, aorta and kidney stones were inoculated into cell culture medium containing 10% fetal bovine serum in the absence or presence of inhibitors of ALP or pyrophosphate. A calcific NP biofilm developed within 1 week after inoculation and their development was reduced by pyrophosphate and inhibitors of ALP. ALP protein and enzymatic activity were detected in washed NPs, whether calcified or decalcified. Therefore, ALP activity is required for the formation of calcifying NPs in vitro, as has previously been implicated during pathological calcification in vivo.

Original languageEnglish (US)
Pages (from-to)1339-1345
Number of pages7
JournalActa Biomaterialia
Volume7
Issue number3
DOIs
StatePublished - Mar 2011

Fingerprint

Phosphatases
Human Development
Nanoparticles
Alkaline Phosphatase
Biomineralization
Kidney Calculi
Biofilms
Carotid Arteries
Cell culture
Culture Media
Aorta
In Vitro Techniques
Catalyst activity
Enzymes
Cell Culture Techniques
Proteins
Serum
Experiments

Keywords

  • Biologic nanoparticles
  • Calcification
  • Heterotopic mineralization
  • Hydroxyapatite
  • Nanobacteria

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Key role of alkaline phosphatase in the development of human-derived nanoparticles in vitro. / Hunter, Larry W.; Shiekh, Farooq A.; Pisimisis, George T.; Kim, Sung Hoon; Edeh, Samuel N.; Miller, Virginia M; Lieske, John C.

In: Acta Biomaterialia, Vol. 7, No. 3, 03.2011, p. 1339-1345.

Research output: Contribution to journalArticle

Hunter, Larry W. ; Shiekh, Farooq A. ; Pisimisis, George T. ; Kim, Sung Hoon ; Edeh, Samuel N. ; Miller, Virginia M ; Lieske, John C. / Key role of alkaline phosphatase in the development of human-derived nanoparticles in vitro. In: Acta Biomaterialia. 2011 ; Vol. 7, No. 3. pp. 1339-1345.
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