Keratinocyte growth factor ameliorates cyclophosphamide-induced ulcerative hemorrhagic cystitis

Thomas R. Ulich, Lane Whitcomb, Winson Tang, Peg O.Conner Tressel, John Tarpley, Eunhee S. Yi, David Lacey

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49 Scopus citations

Abstract

To determine whether keratinocyte growth factor (KGF), an epithelial and urothelial growth factor, ameliorates cyclophosphamide (CP)-induced cystitis in rats, KGF (5 mg/kg) was injected in rats as a single i.v. injection 24 h prior to i.p. injection of CP (200 mg/kg). Bladders were evaluated histologically 48 h after CP injection, and KGF pretreatment was found to almost completely prevent CP-induced ulcerative hemorrhagic cystitis. Urinary KGF levels were measured by ELISA, and KGF was found to be undetectable in control urine, but it was found to appear in the urine of KGF-treated rats at 8 h, with a peak concentration of approximately 10 ng/ml. Bilateral nephrectomy did not diminish the proliferative effect of KGF on urothelium, suggesting that the contribution of urinary KGF to urothelial proliferation is insignificant. In conclusion, systemic administration of KGF is protective against CP-induced cystitis. Although KGF appears in the urine, unirary KGF is not necessary for the proliferative action of KGF on urothelium.

Original languageEnglish (US)
Pages (from-to)472-475
Number of pages4
JournalCancer research
Volume57
Issue number3
StatePublished - Feb 27 1997

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Ulich, T. R., Whitcomb, L., Tang, W., Tressel, P. O. C., Tarpley, J., Yi, E. S., & Lacey, D. (1997). Keratinocyte growth factor ameliorates cyclophosphamide-induced ulcerative hemorrhagic cystitis. Cancer research, 57(3), 472-475.