TY - JOUR
T1 - Keratinocyte growth factor ameliorates cyclophosphamide-induced ulcerative hemorrhagic cystitis
AU - Ulich, Thomas R.
AU - Whitcomb, Lane
AU - Tang, Winson
AU - Tressel, Peg O.Conner
AU - Tarpley, John
AU - Yi, Eunhee S.
AU - Lacey, David
PY - 1997
Y1 - 1997
N2 - To determine whether keratinocyte growth factor (KGF), an epithelial and urothelial growth factor, ameliorates cyclophosphamide (CP)-induced cystitis in rats, KGF (5 mg/kg) was injected in rats as a single i.v. injection 24 h prior to i.p. injection of CP (200 mg/kg). Bladders were evaluated histologically 48 h after CP injection, and KGF pretreatment was found to almost completely prevent CP-induced ulcerative hemorrhagic cystitis. Urinary KGF levels were measured by ELISA, and KGF was found to be undetectable in control urine, but it was found to appear in the urine of KGF-treated rats at 8 h, with a peak concentration of approximately 10 ng/ml. Bilateral nephrectomy did not diminish the proliferative effect of KGF on urothelium, suggesting that the contribution of urinary KGF to urothelial proliferation is insignificant. In conclusion, systemic administration of KGF is protective against CP-induced cystitis. Although KGF appears in the urine, unirary KGF is not necessary for the proliferative action of KGF on urothelium.
AB - To determine whether keratinocyte growth factor (KGF), an epithelial and urothelial growth factor, ameliorates cyclophosphamide (CP)-induced cystitis in rats, KGF (5 mg/kg) was injected in rats as a single i.v. injection 24 h prior to i.p. injection of CP (200 mg/kg). Bladders were evaluated histologically 48 h after CP injection, and KGF pretreatment was found to almost completely prevent CP-induced ulcerative hemorrhagic cystitis. Urinary KGF levels were measured by ELISA, and KGF was found to be undetectable in control urine, but it was found to appear in the urine of KGF-treated rats at 8 h, with a peak concentration of approximately 10 ng/ml. Bilateral nephrectomy did not diminish the proliferative effect of KGF on urothelium, suggesting that the contribution of urinary KGF to urothelial proliferation is insignificant. In conclusion, systemic administration of KGF is protective against CP-induced cystitis. Although KGF appears in the urine, unirary KGF is not necessary for the proliferative action of KGF on urothelium.
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M3 - Article
C2 - 9012476
AN - SCOPUS:0031052385
SN - 0008-5472
VL - 57
SP - 472
EP - 475
JO - Cancer research
JF - Cancer research
IS - 3
ER -