Keratinocyte expression of MMP3 enhances differentiation and prevents tumor establishment

Lisa J. McCawley, Jane Wright, Bonnie J. LaFleur, Howard C. Crawford, Lynn M. Matrisian

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Matrix metalloproteinase (MMP)-3 is induced by multiple cell types in the skin during processes involved in both normal and pathological tissue remodeling. We previously demonstrated that MMP3-null animals have an increased sensitivity to the development of squamous cell carcinoma, suggesting that overall, MMP3 has a protective role in squamous cell carcinoma. However, not all cellular responses affected by a loss of MMP3 are tumor-protective, and tumor expression of MMP3 is co-incident with an invasive tumor phenotype. Transgenic mice were generated with MMP3 targeted to keratinocytes to examine the biological role of tumor-produced MMP3. Overexpression of MMP3 reduced tumor multiplicity in response to chemically induced squamous cell carcinoma. Vascular density was increased with MMP3 overexpression; however, other cellular processes, including tumor growth and leukocyte infiltration, were unaffected. In accordance with the change in tumor multiplicity, SP-1 murine papilloma cell lines that were generated to stably express MMP3 lost the capacity to establish palpable tumors following orthotopic injection into immunocompromised mice. Analysis of epidermal biopsies taken at 1 to 2 weeks postinjection revealed that these MMP3-expressing Sp-1 lines had reduced levels of proliferation and pronounced differentiation. These same cells demonstrated an increased ability to differentiate in vitro, an effect that was inhibited by broad-spectrum MMP and selective MMP3 inhibition. These studies suggest that keratinocyte expression of MMP3 promotes cellular differentiation, impeding tumor establishment during tumorigenesis.

Original languageEnglish (US)
Pages (from-to)1528-1539
Number of pages12
JournalAmerican Journal of Pathology
Volume173
Issue number5
DOIs
StatePublished - Nov 2008
Externally publishedYes

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Keratinocytes
Neoplasms
Squamous Cell Carcinoma
Matrix Metalloproteinase 3
Papilloma
Matrix Metalloproteinases
Transgenic Mice
Blood Vessels
Carcinogenesis
Leukocytes
Phenotype
Biopsy
Cell Line
Skin
Injections
Growth

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

McCawley, L. J., Wright, J., LaFleur, B. J., Crawford, H. C., & Matrisian, L. M. (2008). Keratinocyte expression of MMP3 enhances differentiation and prevents tumor establishment. American Journal of Pathology, 173(5), 1528-1539. https://doi.org/10.2353/ajpath.2008.080132

Keratinocyte expression of MMP3 enhances differentiation and prevents tumor establishment. / McCawley, Lisa J.; Wright, Jane; LaFleur, Bonnie J.; Crawford, Howard C.; Matrisian, Lynn M.

In: American Journal of Pathology, Vol. 173, No. 5, 11.2008, p. 1528-1539.

Research output: Contribution to journalArticle

McCawley, LJ, Wright, J, LaFleur, BJ, Crawford, HC & Matrisian, LM 2008, 'Keratinocyte expression of MMP3 enhances differentiation and prevents tumor establishment', American Journal of Pathology, vol. 173, no. 5, pp. 1528-1539. https://doi.org/10.2353/ajpath.2008.080132
McCawley, Lisa J. ; Wright, Jane ; LaFleur, Bonnie J. ; Crawford, Howard C. ; Matrisian, Lynn M. / Keratinocyte expression of MMP3 enhances differentiation and prevents tumor establishment. In: American Journal of Pathology. 2008 ; Vol. 173, No. 5. pp. 1528-1539.
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