Kappa light chain gene rearrangement in T-cell acute lymphoblastic leukemia

C. A. Hanson, M. Thamilarasan, C. W. Ross, L. M. Stoolman, B. Schnitzer

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Immunoglobulin and T-cell receptor gene rearrangement assays are sensitive methods of detecting clonality in lymphoproliferative disorders. The lack of lineage specificity of immunoglobulin heavy chain and T-cell receptor beta and gamma gene probes in acute leukemia is well established. However, immunoglobulin light chain gene rearrangement traditionally has been considered a highly specific indicator of a clonal B-lineage process. The authors describe a case of T-cell acute lymphoblastic leukemia in which Southern blot hybridization studies showed rearrangement of the T-cell receptor beta chain gene. Unexpectedly, the immunoglobulin kappa light chain gene also was rearranged; no immunoglobulin heavy chanin gene rearrangement was seen. Kappa rearrangement was confirmed with the use of three restriction endonucleases. No rearrangements were seen from normal skin tissue, making a restriction enzyme site polymorphism highly unlikely. Northern blot studies showed a normal-size, T-cell receptor beta chain gene transcript; no immunoglobulin RNA was identified. These results describe the first reported case of kappa light chain gene rearrangement in a T-cell acute lymphoblastic leukemia. The findings emphasize the necessity of interpreting molecular hybridization studies in conjunction with routine morphology and immunophenotyping studies.

Original languageEnglish (US)
Pages (from-to)563-568
Number of pages6
JournalAmerican Journal of Clinical Pathology
Volume93
Issue number4
StatePublished - 1990
Externally publishedYes

Fingerprint

T-Lymphocyte Gene Rearrangement
T-Cell Receptor beta Genes
Gene Rearrangement
Immunoglobulin Light Chain Genes
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
Immunoglobulins
Light
T-Cell Receptor gamma Genes
T-Cell Receptor Genes
Immunophenotyping
Immunoglobulin Heavy Chains
Lymphoproliferative Disorders
DNA Restriction Enzymes
Southern Blotting
Northern Blotting
Leukemia
RNA
Skin
Enzymes

Keywords

  • Flow cytometry
  • Gene rearrangement
  • Kappa
  • T-cell acute lymphoblastic leukemia

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Hanson, C. A., Thamilarasan, M., Ross, C. W., Stoolman, L. M., & Schnitzer, B. (1990). Kappa light chain gene rearrangement in T-cell acute lymphoblastic leukemia. American Journal of Clinical Pathology, 93(4), 563-568.

Kappa light chain gene rearrangement in T-cell acute lymphoblastic leukemia. / Hanson, C. A.; Thamilarasan, M.; Ross, C. W.; Stoolman, L. M.; Schnitzer, B.

In: American Journal of Clinical Pathology, Vol. 93, No. 4, 1990, p. 563-568.

Research output: Contribution to journalArticle

Hanson, CA, Thamilarasan, M, Ross, CW, Stoolman, LM & Schnitzer, B 1990, 'Kappa light chain gene rearrangement in T-cell acute lymphoblastic leukemia', American Journal of Clinical Pathology, vol. 93, no. 4, pp. 563-568.
Hanson CA, Thamilarasan M, Ross CW, Stoolman LM, Schnitzer B. Kappa light chain gene rearrangement in T-cell acute lymphoblastic leukemia. American Journal of Clinical Pathology. 1990;93(4):563-568.
Hanson, C. A. ; Thamilarasan, M. ; Ross, C. W. ; Stoolman, L. M. ; Schnitzer, B. / Kappa light chain gene rearrangement in T-cell acute lymphoblastic leukemia. In: American Journal of Clinical Pathology. 1990 ; Vol. 93, No. 4. pp. 563-568.
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