Kappa light chain gene rearrangement in T-cell acute lymphoblastic leukemia

C. A. Hanson, M. Thamilarasan, C. W. Ross, L. M. Stoolman, B. Schnitzer

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Immunoglobulin and T-cell receptor gene rearrangement assays are sensitive methods of detecting clonality in lymphoproliferative disorders. The lack of lineage specificity of immunoglobulin heavy chain and T-cell receptor beta and gamma gene probes in acute leukemia is well established. However, immunoglobulin light chain gene rearrangement traditionally has been considered a highly specific indicator of a clonal B-lineage process. The authors describe a case of T-cell acute lymphoblastic leukemia in which Southern blot hybridization studies showed rearrangement of the T-cell receptor beta chain gene. Unexpectedly, the immunoglobulin kappa light chain gene also was rearranged; no immunoglobulin heavy chanin gene rearrangement was seen. Kappa rearrangement was confirmed with the use of three restriction endonucleases. No rearrangements were seen from normal skin tissue, making a restriction enzyme site polymorphism highly unlikely. Northern blot studies showed a normal-size, T-cell receptor beta chain gene transcript; no immunoglobulin RNA was identified. These results describe the first reported case of kappa light chain gene rearrangement in a T-cell acute lymphoblastic leukemia. The findings emphasize the necessity of interpreting molecular hybridization studies in conjunction with routine morphology and immunophenotyping studies.

Original languageEnglish (US)
Pages (from-to)563-568
Number of pages6
JournalAmerican journal of clinical pathology
Volume93
Issue number4
DOIs
StatePublished - Jan 1 1990

Keywords

  • Flow cytometry
  • Gene rearrangement
  • Kappa
  • T-cell acute lymphoblastic leukemia

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Hanson, C. A., Thamilarasan, M., Ross, C. W., Stoolman, L. M., & Schnitzer, B. (1990). Kappa light chain gene rearrangement in T-cell acute lymphoblastic leukemia. American journal of clinical pathology, 93(4), 563-568. https://doi.org/10.1093/ajcp/93.4.563