Kallikrein-mediated cell signalling: Targeting proteinase-activated receptors (PARs)

Katerina Oikonomopoulou, Kristina K. Hansen, Mahmoud Saifeddine, Nathalie Vergnolle, Illa Tea, Michael Blaber, Sachiko I. Blaber, Isobel Scarisbrick, Eleftherios P. Diamandis, Morley D. Hollenberg

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

We tested the hypothesis that human tissue kallikreins (hKs) may regulate signal transduction by cleaving and activating proteinase-activated receptors (PARs). We found that hK5, 6 and 14 cleaved PAR N-terminal peptide sequences representing the cleavage/activation motifs of human PAR1 and PAR2 to yield receptor-activating peptides. hK5, 6 and 14 activated calcium signalling in rat PAR2-expressing (but not background) KNRK cells. Calcium signalling in HEK cells co-expressing human PAR1 and PAR2 was also triggered by hK14 (via PAR1 and PAR 2) and hK6 (via PAR2). In isolated rat platelets that do not express PAR1, but signal via PAR4, hK14 also activated PAR-dependent calcium signalling responses and triggered aggregation. The aggregation response elicited by hK14 was in contrast to the lack of aggregation triggered by hK5 and 6. hK14 also caused vasorelaxation in a phenylephrine-preconstricted rat aorta ring assay and triggered oedema in an in vivo model of murine paw inflammation. We propose that, like thrombin and trypsin, the kallikreins must now be considered as important 'hormonal' regulators of tissue function, very likely acting in part via PARs.

Original languageEnglish (US)
Pages (from-to)817-824
Number of pages8
JournalBiological Chemistry
Volume387
Issue number6
DOIs
StatePublished - Jun 1 2006

Keywords

  • Inflammation
  • Kallikreins
  • Receptors
  • Serine proteinases
  • Signal transduction
  • Trypsin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Clinical Biochemistry

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    Oikonomopoulou, K., Hansen, K. K., Saifeddine, M., Vergnolle, N., Tea, I., Blaber, M., Blaber, S. I., Scarisbrick, I., Diamandis, E. P., & Hollenberg, M. D. (2006). Kallikrein-mediated cell signalling: Targeting proteinase-activated receptors (PARs). Biological Chemistry, 387(6), 817-824. https://doi.org/10.1515/BC.2006.104