K-ras-2 and p53 genotyping of intestinal-type adenocarcinoma of the nasal cavity and paranasal sinuses

Tsung Teh Wu, Leon Barnes, Anke Bakker, Patricia A. Swalsky, Sydney D. Finkelstein

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Intestinal-type adenocarcinoma (ITAC) of the nasal cavity and paranasal sinuses is an uncommon neoplasm manifesting a variety of histologic growth patterns, most of which are reminiscent of colorectal adenocarcinoma To determine whether this morphologic resemblance is mirrored in equivalent genetic alterations, a series of ITAC was genotyped using archival, formalin-fixed tissues for point mutational damage in K-ras-2 oncogene and p53 tumor suppressor gene, forms of DNA damage frequently found in colorectal adenocarcinoma In contrast to colorectal adenocarcinoma, which demonstrates K-ras-2 mutation in about 50% of cases, ITAC showed no evidence of K-ras-2 mutation. p53 mutations were present in 18% of ITAC (2 of 11) compared to more than 75% incidence of p53 mutation seen in colorectal adenocarcinoma Fifty-eight percent of ITAC demonstrated scattered positive p53 immunohistochemical nuclear staining, but no mutations were identified in exon-5 through exon-8 by genotyping. There was no specific p53 alterations with respect to histologic subtype of ITAC. Although ITAC and colorectal adenocarcinoma are histologically similar, there are important differences at the genetic level based on expression of K-ras-2 and pS3 abnormalities.

Original languageEnglish (US)
Pages (from-to)199-204
Number of pages6
JournalModern Pathology
Volume9
Issue number3
StatePublished - Mar 1996
Externally publishedYes

Fingerprint

Paranasal Sinuses
Nasal Cavity
Adenocarcinoma
Mutation
Exons
ras Genes
Tumor Suppressor Genes
Formaldehyde
DNA Damage
Staining and Labeling

Keywords

  • Intestinal-type adenocarcinoma
  • K-ras
  • Nasal cavity
  • P53
  • Paranasal sinus

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Wu, T. T., Barnes, L., Bakker, A., Swalsky, P. A., & Finkelstein, S. D. (1996). K-ras-2 and p53 genotyping of intestinal-type adenocarcinoma of the nasal cavity and paranasal sinuses. Modern Pathology, 9(3), 199-204.

K-ras-2 and p53 genotyping of intestinal-type adenocarcinoma of the nasal cavity and paranasal sinuses. / Wu, Tsung Teh; Barnes, Leon; Bakker, Anke; Swalsky, Patricia A.; Finkelstein, Sydney D.

In: Modern Pathology, Vol. 9, No. 3, 03.1996, p. 199-204.

Research output: Contribution to journalArticle

Wu, TT, Barnes, L, Bakker, A, Swalsky, PA & Finkelstein, SD 1996, 'K-ras-2 and p53 genotyping of intestinal-type adenocarcinoma of the nasal cavity and paranasal sinuses', Modern Pathology, vol. 9, no. 3, pp. 199-204.
Wu TT, Barnes L, Bakker A, Swalsky PA, Finkelstein SD. K-ras-2 and p53 genotyping of intestinal-type adenocarcinoma of the nasal cavity and paranasal sinuses. Modern Pathology. 1996 Mar;9(3):199-204.
Wu, Tsung Teh ; Barnes, Leon ; Bakker, Anke ; Swalsky, Patricia A. ; Finkelstein, Sydney D. / K-ras-2 and p53 genotyping of intestinal-type adenocarcinoma of the nasal cavity and paranasal sinuses. In: Modern Pathology. 1996 ; Vol. 9, No. 3. pp. 199-204.
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